Human Phospho-p70 S6 Kinase (T389) Antibody

Catalog #: MAB8963
2 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8963
MAB8963-SP

Detection of Human Phospho-p70 S6 Kinase (T389)/p85 S6 Kinase (T412) by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-p70 S6 Kinase (T389) Antibody Summary

Species Reactivity

Human

Specificity

Detects human p70 S6 Kinase/p85 S6 Kinase when phosphorylated at T389/T412, respectively.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1045C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human p70 S6 Kinase T389 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunocytochemistry

2-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-p70 S6 Kinase (T389)/p85 S6 Kinase (T412) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T389) and Phospho-p85 S6 Kinase (T412) at approximately 70 and 85 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-p70 S6 Kinase (T389) in MCF‑7 Human Cell Line. p70 S6 Kinase phosphorylated at T389 was detected in immersion fixed serum starved MCF-7 human breast cancer cell line untreated (lower panel) or treated with Recombinant Human IGF-I (Catalog # 291-G1) using Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases
20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as T389, a site phosphorylated by Phosphoinositide-Dependent Kinase 1 (PDK1).

References

Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human Phospho-p70 S6 Kinase (T389) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Characterizing the distributions of IDO-1 expressing macrophages/microglia in human and murine brains and evaluating the immunological and physiological roles of IDO-1 in RAW264.7/BV-2 cells
Authors: R Ji, L Ma, X Chen, R Sun, L Zhang, H Saiyin, W Wei
PLoS ONE, 2021-11-04;16(11):e0258204.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Effect of gut microbiota on depressive-like behaviors in mice is mediated by the endocannabinoid system
Authors: G Chevalier, E Siopi, L Guenin-Mac, M Pascal, T Laval, A Rifflet, IG Boneca, C Demangel, B Colsch, A Pruvost, E Chu-Van, A Messager, F Leulier, G Lepousez, G Eberl, PM Lledo
Nature Communications, 2020-12-11;11(1):6363.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot