Human Phospho-Paxillin (Y118) Antibody

Catalog #: MAB6164 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6164
MAB6164-SP

Detection of Human Phospho-Paxillin (Y118) by Western Blot.

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Human Phospho-Paxillin (Y118) Antibody Summary

Species Reactivity

Human

Specificity

Detects Phospho-Paxillin (Y118) in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 601327

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human Paxillin Y118 site
Accession # P49023

Formulation

Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-Paxillin (Y118) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate for 5 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y118) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6164) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Paxillin (Y118) at approximately 80 kDa (as indicated). For additional reference, the membran was stripped and reprobed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (lowerpanel, Catalog # AF4259). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: Paxillin

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Entrez Gene IDs

5829 (Human); 19303 (Mouse); 360820 (Rat)

Alternate Names

FLJ16691; Paxillin; PXN