Human Phospho-PLC-gamma 2 (Y759) Antibody

Catalog #: MAB7377 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7377
MAB7377-SP

Detection of Human Phospho-PLC‑ gamma 2 (Y759) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-PLC-gamma 2 (Y759) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-PLC-gamma 2 (Y759) in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 744757

Purification

Protein A or G purified from ascites

Immunogen

Phosphopeptide containing the human PLC-gamma 2 Y759 site
Accession # P16885

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from ascites

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-PLC‑ gamma 2 (Y759) by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes or 10 µg/mL Goat Anti-Human IgM µ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-PLC- gamma2 (Y759) at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospho-PLC‑ gamma 2 (Y759) in Ramos Human Cell Line. PLC- gamma2 phosphorylated at Y759 was detected in immersion fixed Ramos human Burkitt's lymphoma cell line treated (upper panel) or untreated (lower panel) with pervanadate for 5 minutes using Mouse Anti-Human Phospho-PLC- gamma2 (Y759) Monoclonal Antibody (Catalog # MAB7377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-gamma 2

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC-gamma 2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones, and downstream through Lck kinase-dependent phosphorylation at Y753 and Y759. Activated PLC-gamma 2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes. Human PLC-gamma 2 shares 94% and 95% sequence identity overall with mouse and rat PLC-gamma 2, respectively, and 100% sequence identity within the peptide immunogen.

Long Name

Phospholipase C gamma 2

Entrez Gene IDs

5336 (Human); 234779 (Mouse); 29337 (Rat)

Alternate Names

EC 3.1.4.11; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); Phospholipase C-gamma-2,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phospholipase C-IV; PLCG2; PLCgamma 2; PLC-gamma 2; PLC-gamma-2; PLC-IV