Human PLA2G2A Antibody

Catalog #: AF5374 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5374
AF5374-SP

All PLA2G2A Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PLA2G2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human PLA2G2A in direct ELISAs and Western blots. In Western blots, approximately 60% cross-reactivity with recombinant mouse PLA2G2A is observed and less than 5% cross-reactivity with recombinant human (rh) PLA2G1B and rhPLA2G7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PLA2G2A
Asn21-Cys144
Accession # P14555

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Phospholipase A2 Group IIA/PLA2G2A (Catalog # 5374-PL)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Phospholipase A2 Group IIA/PLA2G2A (Catalog # 5374-PL), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLA2G2A

Secretory phopholipase A₂ is an enzyme that hydrolyzes the sn-2 ester bond of phospholipids, generating non-esterified free fatty acids and lysophospholipids (1‑4). Most secretory PLA₂s are stored in cytoplasmic granules and are released in the extracellular environment on appropriate cell activation. Thus, they are present at higher concentration in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic disease, such as acute pancreatitis, rheumatoid arthritis, bronchial asthma, and allergic rhinitis. PLA2G2A is a calcium dependent phospholipase expressed in many cell types associated with inflammation, including platelets, neutrophils, and mast cells. It has been recognized as an acute phase reactant and its concentration increases during systemic inflammation such as sepsis, rheumatoid arthritis, and cardiovascular disease (5). It may function as an enzymatic component of the host defense mechanism. For example, human tears contain a high concentration of PLA2G2A and this is the principal bactericidal factor against Gram-positive bacteria in this fluid (1). It may play a role in cell proliferation through binding a receptor on membrane surface or extracellular matrix components such as heparan sulfate proteoglycan and integrins (6). It has been shown to have pro-atherogenic properties both in the circulation and within the arterial wall (7). Circulating levels of PLA2G2A are higher in coronary artery disease (CAD) patients and are associated with increased risk of future CAD (8).

References

Lambeau, G. and M.H. Gelb (2008) Ann. Rev. Biochem. 77:495.
Webb, N.R. (2005) Curr. Opin. Lipid. 16:341.
Triggiani, M. et al. (2005) J. Allergy Clin. Immunol. 116:1000.
Murakami, M. and I. Kudo (2004) Biol. Pharm. Bull. 27:1158.
Crowl, R.M. et al. (1991) J. Biol. Chem. 266:2647.
Saegusa, J. et al. (2008) J. Biol. Chem. 283:26107.
de Beer, F.C. and N.R. Webb (2006) Arterioscler. Thromb. Vasc. Biol. 6:1421.
Wootton, P.T.E. et al. (2006) Human Mol. Genet. 15:355.

Long Name

Phospholipase A2 Group IIA

Entrez Gene IDs

5320 (Human); 18780 (Mouse); 29692 (Rat)

Alternate Names

EC 3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; Mom1; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLAS1; RASF-A; sPLA2