Human PLD2 Antibody

Catalog #: MAB10123 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10123-100
MAB10123-SP

Detection of Human PLD2 by Western Blot.

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Human PLD2 Antibody Summary

Species Reactivity

Human

Specificity

Detects huma PLD2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 846224

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PLD2
Met1-His117
Accession # O14939

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PLD2 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PLD2 Monoclonal Antibody (Catalog # MAB10123) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PLD2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLD2

Phospholipase D2 (PLD2) is an enzyme encoded by the PLD2 gene. Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. The Phospholipase D (PLD) lipid-signaling enzyme superfamily has long been studied for its roles in cell communication and a wide range of cell biological processes. Potent PLD2 inhibitors are available, like VU 0364739 hydrochloride (Tocris Cat. 4171) and ML 298 hydrochloride (Tocris Cat. 4895). Three different PLD2 isoforms -PLD2A, PLD2B and PLD2C- have been described, ranging from 38 to 105 KDa.

Long Name

Phospholipase D2

Entrez Gene IDs

5338 (Human); 18806 (Mouse); 25097 (Rat)

Alternate Names

Choline Phosphatase 2; HPLD2; PLD1C; PLD2

Product Datasheets

Product Datasheet

COA

SDS

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