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Human PlGF Quantikine HS ELISA Kit

Catalog #: HSPG00
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
HSPG00
Control Products Available
QC144: Quantikine Immunoassay Control Set 813 for Human HS PlGF
Human PlGF ELISA Standard Curve
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Product Details
Procedure
Citations (4)
FAQs
Supplemental Products
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human PlGF Quantikine HS ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
0.326 pg/mL
Assay Range
3.1 - 200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human PlGF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine HS Human PlGF Immunoassay is a 3.5 hour solid phase
ELISA designed to measure PlGF levels in serum and plasma. It contains
E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 27.2 57.4 108 27.3 57.7 108
Standard Deviation 0.72 1.87 2.84 1.65 3.88 7.37
CV% 2.6 3.3 2.6 6 6.7 6.8

Recovery

The recovery of PlGF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 98 85-115
Heparin Plasma (n=4) 90 86-97
Serum (n=4) 92 86-97

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of PlGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PlGF ELISA Linearity

Scientific Data

N/A Human PlGF ELISA Standard Curve View Larger

Human PlGF ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is a homodimeric growth factor that competes with VEGF for binding to VEGF R1/Flt-1. It therefore increases the availability of VEGF to bind to VEGF R2/KDR/Flk-1 and trigger angiogenesis. It can form heterodimers with some forms of VEGF and decrease the angiogenic effect of VEGF on VEGF R2. Circulating PlGF levels increase during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. PlGF induces monocyte activation and migration as well as production of inflammatory cytokines and VEGF. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. PlGF can also inhibit TIMP3 expression in the spleen, leading to immune triggering of hypertension.

Long Name:
Placenta Growth Factor
Entrez Gene IDs:
5228 (Human); 18654 (Mouse)
Alternate Names:
D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  5.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 6 times.
    8. 50 µL Substrate Solution
  8.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Protect from light. Do not wash the plate.
    9. 50 µL Amplifier Solution
  9.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.

Citations for Human PlGF Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Dynamics of Early Signalling Events during Fracture Healing and Potential Serum Biomarkers of Fracture Non-Union in Humans
    Authors: AN Burska, PV Giannoudis, BH Tan, D Ilas, E Jones, F Ponchel
    J Clin Med, 2020-02-11;9(2):.
    Species: Human
    Sample Types: Serum
  2. Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles
    Authors: G Merckx, B Hosseinkha, S Kuypers, S Deville, J Irobi, I Nelissen, L Michiels, I Lambrichts, A Bronckaers
    Cells, 2020-01-28;9(2):.
    Species: Human
    Sample Types: Serum
  3. Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation
    Authors: M Perrotta, A Lori, L Carnevale, S Fardella, G Cifelli, R Iacobucci, F Mastroiaco, D Iodice, F Pallante, M Storto, G Lembo, D Carnevale
    Cardiovasc. Res., 2018-03-01;114(3):456-467.
    Species: Mouse
    Sample Types: Tissue Extracts
  4. Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease
    Authors: JM Gu, S Yuan, D Sim, K Abe, P Liu, M Rosenbruch, P Bringmann, K Kauser
    Exp. Hematol., 2018-01-11;0(0):.
    Species: Human
    Sample Types: Plasma

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