Human/Primate EGF Antibody Summary
Applications
Human/Primate EGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![EGF antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). EGF antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/EGF_MAB636_ELISA_Capture_17335.jpg)
EGF in Human Lung Cancer Tissue. EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.
Product Datasheets
Citations for Human/Primate EGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
J. Immunol. Methods, 2012-02-17;378(1):72-80.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light.
Authors: Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F
J. Biotechnol., 2006-04-03;125(2):159-72.
Species: Human
Sample Types: Cell Lysates, Tissue Homogenates
Applications: ELISA Development, Western Blot
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