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Human/Primate IL-17/IL-17A Antibody

Catalog #: MAB317
22 Citations 3 Reviews Datasheet / COA / SDS
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MAB317-SP
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IL‑17/IL‑17A in Human Crohn's Disease Intestine.
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Citations (22)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-17/IL-17A Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
Monoclonal Mouse IgG2B Clone # 41809
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below
Immunoprecipitation
3 µg/100 µg cell lysate
See below

Human/Primate IL-17 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate IL‑17/IL‑17A Biotinylated Antibody (Catalog # BAF317)

Standard: Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)

Neutralization
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-17/IL-17A antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P). View Larger

IL‑17/IL‑17A in Human Crohn's Disease Intestine. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human/Primate IL-17/IL-17A Monoclonal Antibody (Catalog # MAB317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation Immunoprecipitation of Human IL‑17. View Larger

Immunoprecipitation of Human IL‑17. Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Neutralization IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. View Larger

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317). The ND50 is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

IL-17 is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Human/Primate IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Establishment of a humanized animal model of systemic sclerosis in which T helper-17 cells from patients with systemic sclerosis infiltrate and cause fibrosis in the lungs and skin
    Authors: MJ Park, Y Park, JW Choi, JA Baek, HY Jeong, HS Na, YM Moon, ML Cho, SH Park
    Experimental & Molecular Medicine, 2022-09-29;54(9):1577-1585.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
    Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
    Sci Rep, 2020-10-15;10(1):17444.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation
    Authors: J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham
    Cell Rep, 2020-06-30;31(13):107820.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture
  4. Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis
    Authors: Y Liao, J Zhao, K Bulek, F Tang, X Chen, G Cai, S Jia, PL Fox, E Huang, TT Pizarro, MF Kalady, MW Jackson, S Bao, GC Sen, GR Stark, CJ Chang, X Li
    Nat Commun, 2020-02-14;11(1):900.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis
    Authors: Y Liao, J Zhao, K Bulek, F Tang, X Chen, G Cai, S Jia, PL Fox, E Huang, TT Pizarro, MF Kalady, MW Jackson, S Bao, GC Sen, GR Stark, CJ Chang, X Li
    Nat Commun, 2020;11(1):900.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models
    Authors: JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar
    Cells, 2019-09-15;8(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  7. Serial Monitoring of Immune Markers Being Represented Regulatory T Cell/T Helper 17 Cell Ratio: Indicating Tolerance for Tapering Immunosuppression after Liver Transplantation
    Authors: J Jhun, SH Lee, SK Lee, HY Kim, ES Jung, DG Kim, J Choi, SH Bae, SK Yoon, BH Chung, CW Yang, ML Cho, JY Choi
    Front Immunol, 2018-03-01;9(0):352.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  8. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  9. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  10. Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis
    Authors: Dobrina N Hull
    Arthritis Res. Ther, 2016-12-23;18(1):303.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  11. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
    Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
    Eur J Immunol, 2015-12-14;46(2):440-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013-10-12;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  13. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2009-11-04;127(2):150-6.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: ELISA Development, IHC-P
  15. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009-03-01;182(0):3112-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils.
    Authors: Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J
    J. Invest. Dermatol., 2008-10-09;129(3):671-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008-09-15;181(6):4299-310.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
    Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
    J. Immunol., 2008-01-01;180(1):655-63.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  20. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  21. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004-07-15;173(2):1463-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization

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Reviews for Human/Primate IL-17/IL-17A Antibody

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Human/Primate IL-17/IL-17A Antibody
By Anonymous on 08/19/2021
Application: IHC Sample Tested: Ischemic brain tissue Species: Human
Immunohistochemistry Human/Primate IL-17/IL-17A Antibody MAB317
Human/Primate IL-17/IL-17A Antibody
By Anonymous on 08/22/2018
Application: ELISA Sample Tested: Cell culture supernatant Species: Human

Use the antibody to coat the wells in an immunoassay.

ELISA Human/Primate IL-17/IL-17A Antibody MAB317
Human/Primate IL-17/IL-17A Antibody
By Balaji Mahender on 01/18/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human