Home / IL-6 / Human/Primate IL-6 Antibody

Human/Primate IL-6 Antibody

Catalog #: MAB206
77 Citations 14 Reviews Datasheet / COA / SDS
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MAB206-SP
MAB206-100
MAB206-500
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Detection of Recombinant Human IL‑6 by Western Blot.
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Citations (77)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-6 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL‑6, rhOSM, rhLIF, rhIL‑11, rhgp130, or rhCNTF.
Source
Monoclonal Mouse IgG1 Clone # 6708
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Human/Primate IL-6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate IL‑6 Biotinylated Antibody (Catalog # BAF206)

Standard: Recombinant Human IL-6 Protein (Catalog # 206-IL)

Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 8.00 - 80.0 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human IL-6 antibody by Western Blot. View Larger

Detection of Recombinant Human IL‑6 by Western Blot. Western blot shows 25 ng of Recombinant Human IL-6 (Catalog # 206-IL), Recombinant Mouse IL-6 (Catalog # 406-ML) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/ Primate IL-6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry IL-6 antibody in Human Skin by Immunohistochemistry (IHC-Fr). View Larger

IL‑6 in Human Skin. IL-6 was detected in immersion fixed frozen sections of hyperplastic human skin using Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206). The ND50 is typically 8.00 - 80.0 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Human/Primate IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

77 Citations: Showing 1 - 10
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  1. Maternal pre-eclampsia serum increases neurite growth and mitochondrial function through a potential IL-6-dependent mechanism in differentiated SH-SY5Y cells
    Authors: A Barron, S Manna, CJ McElwain, A Musumeci, FP McCarthy, GW O'Keeffe, CM McCarthy
    Frontiers in Physiology, 2023-01-12;13(0):1043481.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. A portable analog front-end system for label-free sensing of proteins using nanowell array impedance sensors
    Authors: M Tayyab, P Xie, MA Sami, H Raji, Z Lin, Z Meng, SR Mahmoodi, M Javanmard
    Scientific Reports, 2022-11-22;12(1):20119.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  3. IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact
    Authors: W Singhatana, S Kitpakorns, M Toso, P Pavasant
    Royal Society open science, 2022-07-27;9(7):220056.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses
    Authors: V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg
    Cell Reports, 2022-04-19;39(3):110727.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization
    Authors: K Ming-Chin, AA Achuthan, DP De Souza, TJ Lupancu, KJ Binger, MKS Lee, Y Xu, MJ McConville, NA de Weerd, D Dragoljevi, PJ Hertzog, AJ Murphy, JA Hamilton, AJ Fleetwood
    Cell Reports, 2022-04-19;39(3):110719.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Colorectal cancer-associated fibroblasts promote metastasis by up-regulating LRG1 through stromal IL-6/STAT3 signaling
    Authors: B Zhong, B Cheng, X Huang, Q Xiao, Z Niu, YF Chen, Q Yu, W Wang, XJ Wu
    Cell Death & Disease, 2021-12-20;13(1):16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Dynamic interplay of two molecular switches enabled by the MEK1/2-ERK1/2 and IL-6-STAT3 signaling axes controls epithelial cell migration in response to growth factors
    Authors: L Qin, X Cao, T Kaneko, C Voss, X Liu, G Wang, SS Li
    The Journal of Biological Chemistry, 2021-09-02;0(0):101161.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Functional inhibition of cancer stemness-related protein DPP4 rescues tyrosine kinase inhibitor resistance in renal cell carcinoma
    Authors: S Kamada, T Namekawa, K Ikeda, T Suzuki, M Kagawa, H Takeshita, A Yano, K Okamoto, T Ichikawa, K Horie-Inou, S Kawakami, S Inoue
    Oncogene, 2021-05-10;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Interleukin-6 participates in human pancreatic stellate cell activation and collagen I production via TGF-&beta1/Smad pathway
    Authors: M Zheng, H Li, L Sun, DR Brigstock, R Gao
    Cytokine, 2021-04-21;0(0):155536.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinoma
    Authors: T Seki, N Yanaihara, JS Shapiro, M Saito, J Tabata, R Yokomizo, D Noguchi, T Kuroda, A Kawabata, J Suzuki, K Takahashi, H Matsuzawa, M Miyake, M Takenaka, Y Iida, S Yanagida, A Okamoto
    Scientific Reports, 2021-04-08;11(1):7689.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Interleukin-1&alpha Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation
    Authors: K Bernau, JP Leet, H Floerke, EM Bruhn, AL Noll, IS McDermott, S Esnault, NN Jarjour, N Sandbo
    Cells, 2021-03-02;10(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  12. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
    Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
    Sci Rep, 2020-10-15;10(1):17444.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  13. Suppressive myeloid cells are expanded by biliary tract cancer-derived cytokines in vitro and associate with aggressive disease
    Authors: MB Ware, MY Zaidi, J Yang, MK Turgeon, A Krasinskas, TA Mace, K Keenan, MR Farren, AN Ruggieri, Y Li, C Zhang, Z Chen, GS Young, O Elnaggar, Z Che, SK Maithel, T Bekaii-Saa, B El-Rayes, GB Lesinski
    Br. J. Cancer, 2020-08-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Shikonin blocks human lung adenocarcinoma cell migration and invasion in the inflammatory microenvironment via the IL?6/STAT3 signaling pathway
    Authors: T Pan, F Zhang, F Li, X Gao, Z Li, X Li, X Ren
    Oncol. Rep., 2020-07-09;44(3):1049-1063.
    Species: Human
    Sample Types: Cells, Whole Cells
    Applications: B/N, Neutralization
  15. Mycobacterium tuberculosis Lipoarabinomannan Activates Human Neutrophils via a TLR2/1 Mechanism Distinct from Pam3CSK4
    Authors: JS Hook, M Cao, K Weng, N Kinnare, JG Moreland
    J. Immunol., 2019-12-23;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  16. Tumor suppressor HIC1 is synergistically compromised by cancer-associated fibroblasts and tumor cells through the IL-6/pSTAT3 axis in breast cancer
    Authors: X Sun, Q Qu, Y Lao, M Zhang, X Yin, H Zhu, Y Wang, J Yang, J Yi, M Hao
    BMC Cancer, 2019-12-03;19(1):1180.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  17. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Authors: A Stumpf, T Brandstett, J Hübner, J Rühe
    PLoS ONE, 2019-12-02;14(12):e0225525.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  18. Early loss of Histone H2B monoubiquitylation alters chromatin accessibility and activates key immune pathways that facilitate progression of ovarian cancer
    Authors: J Hooda, M Novak, MP Salomon, C Matsuba, RI Ramos, E MacDuffie, M Song, MS Hirsch, J Lester, V Parkash, BY Karlan, M Oren, DS Hoon, R Drapkin
    Cancer Res., 2018-12-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Exposure to wild-type AAV drives distinct capsid immunity profiles in humans
    Authors: K Kuranda, P Jean-Alpho, C Leborgne, R Hardet, F Collaud, S Marmier, H Costa Verd, G Ronzitti, P Veron, F Mingozzi
    J. Clin. Invest., 2018-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018-08-15;9(1):3267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies
    Authors: C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan
    J. Leukoc. Biol., 2018-07-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
    Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
    Psychoneuroendocrinology, 2018-07-13;9(1):2705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis
    Authors: H Touil, A Kobert, N Lebeurrier, A Rieger, P Saikali, C Lambert, L Fawaz, CS Moore, A Prat, J Gommerman, JP Antel, Y Itoyama, I Nakashima, A Bar-Or
    J Neuroinflammation, 2018-04-19;15(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Age-Related Changes to Human Tear Composition
    Authors: A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini
    Invest. Ophthalmol. Vis. Sci., 2018-04-01;59(5):2024-2031.
    Species: Human
    Sample Types: Tears
  25. Diabetes mellitus secondary to pancreatic diseases (type 3c): The effect of smoking on the exocrine-endocrine interactions of the pancreas
    Authors: M ?liwi?ska-, S Milnerowic, H Milnerowic
    Diab Vasc Dis Res, 2018-03-21;0(0):1479164118764.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  26. Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets
    Authors: S Galland, J Vuille, P Martin, I Letovanec, A Caignard, G Fregni, I Stamenkovi
    Cell Rep, 2017-09-19;20(12):2891-2905.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  28. Sensitization of EGFR wild-type non-small cell lung cancer cells to EGFR-tyrosine kinase inhibitor erlotinib
    Authors: J Raimbourg, MP Joalland, M Cabart, L de Plater, F Bouquet, A Savina, D Decaudin, J Bennouna, FM Vallette, L Lalier
    Mol. Cancer Ther., 2017-05-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line
    Authors: P Ortiz-Mont, A Londoño-Va, JP Vernot
    Cell Commun. Signal, 2017-05-04;15(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Interleukin?6 induces an epithelial?mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration
    Authors: J Zhou, C Zhang, J Pan, L Chen, ST Qi
    Mol Med Rep, 2017-05-02;15(6):4123-4131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Adipogenic differentiation of MSC alters their immunomodulatory properties in a tissue-specific manner
    Authors: H Munir, LS Ward, L Sheriff, S Kemble, S Nayar, F Barone, GB Nash, HM McGettrick
    Stem Cells, 2017-04-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  32. Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway
    Authors: D Wang, S Tang, Q Zhang
    Oncol Lett, 2017-04-21;13(6):4875-4881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  34. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling
    Authors: S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat
    J. Biol. Chem, 2017-03-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017-02-16;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. Monocyte Subsets Coregulate Inflammatory Responses by Integrated Signaling through TNF and IL-6 at the Endothelial Cell Interface
    Authors: M Chimen, CM Yates, HM McGettrick, LS Ward, MJ Harrison, B Apta, LH Dib, BA Imhof, P Harrison, GB Nash, GE Rainger
    J. Immunol, 2017-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
    Authors: HC Hsia, JE Hutti, AS Baldwin
    J. Biol. Chem, 2017-02-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Bone metastasis of prostate cancer can be therapeutically targeted at the TBX2-WNT signaling axis
    Authors: S Nandana, M Tripathi, P Duan, CY Chu, R Mishra, C Liu, R Jin, H Yamashita, M Zayzafoon, NA Bhowmick, HE Zhau, RJ Matusik, LW Chung
    Cancer Res, 2017-01-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016-11-15;90(0):88-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  40. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium
    PLoS ONE, 2016-05-12;11(5):e0155161.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  41. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
    Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
    J Biol Chem, 2015-07-24;290(36):22212-24.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  42. CXCL8 and CCL20 Enhance Osteoclastogenesis via Modulation of Cytokine Production by Human Primary Osteoblasts.
    Authors: Pathak J, Bakker A, Verschueren P, Lems W, Luyten F, Klein-Nulend J, Bravenboer N
    PLoS ONE, 2015-06-23;10(6):e0131041.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  43. Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
    Authors: Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S
    Cancer Res, 2015-05-07;75(13):2629-40.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  44. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
    Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
    J Neurosci, 2015-02-11;35(6):2452-64.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  45. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
    Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
    Endocrinology, 2014-11-26;156(2):670-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  46. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
    Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
    J Immunol, 2014-05-28;193(1):234-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  47. Epstein-Barr virus infection induces indoleamine 2,3-dioxygenase expression in human monocyte-derived macrophages through p38/mitogen-activated protein kinase and NF-kappaB pathways: impairment in T cell functions.
    Authors: Liu W, Lin Y, Xiao H, Xing S, Chen H, Chi P, Zhang G
    J Virol, 2014-04-02;88(12):6660-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014-01-21;111(6):2110-5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Microfluidic Assay
  49. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013-10-12;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  50. Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner.
    Authors: Mace T, Ameen Z, Collins A, Wojcik S, Mair M, Young G, Fuchs J, Eubank T, Frankel W, Bekaii-Saab T, Bloomston M, Lesinski G
    Cancer Res, 2013-03-20;73(10):3007-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  51. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012-07-03;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  52. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  53. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 2011-09-01;26(9):1132-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  54. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011-02-23;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  55. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010-05-01;28(5):939-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  56. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  57. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  58. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009-05-01;39(5):1221-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  59. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
    Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
    J. Immunol., 2008-08-15;181(4):2772-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  60. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008-07-17;43(2):187-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  61. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008-07-01;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  62. C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome.
    Authors: Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD
    J. Immunol., 2008-06-01;180(11):7368-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  63. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
    Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
    AIDS Res. Hum. Retroviruses, 2008-05-01;24(5):667-71.
    Species: Human
    Sample Types: Vaginal Fluid
    Applications: ELISA Development
  64. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
    Authors: Kurago ZB, Lam-ubol A, Stetsenko A
    Head Neck Pathol, 2008-03-01;2(1):1-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  65. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007-10-25;26(1):279-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  66. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
    Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
    J. Clin. Oncol., 2007-10-10;25(29):4542-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  67. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  68. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007-05-01;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  69. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006-07-01;54(7):2096-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  70. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006-02-02;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  71. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
    Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
    Acta Obstet Gynecol Scand, 2005-06-01;84(6):551-7.
    Species: Human
    Sample Types: Amniotic Fluid
    Applications: ELISA Development
  72. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  73. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004-06-01;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  74. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  75. Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation.
    Authors: McLoughlin RM, Witowski J, Robson RL, Wilkinson TS, Hurst SM, Williams AS, Williams JD, Rose-John S, Jones SA, Topley N
    J. Clin. Invest., 2003-08-01;112(4):598-607.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  76. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  77. The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
    Authors: Hess S, 2019, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
    7046, 2001-10-01;44(10):2392-401.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

  1. Which R&D Systems antibody product could be used as an isotype control for catalog # MAB206?

    • Catalog # MAB002 or MAB002R (Mouse IgG1 isotype control) could be used as a control for catalog # MAB206.

  2. What is the light chain of Human/Primate IL-6 Antibody, Catalog # MAB206, Clone # 6708?

    • Catalog # MAB206 has a kappa light chain.

View all Antibody FAQs
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Reviews for Human/Primate IL-6 Antibody

Average Rating: 4.5 (Based on 14 Reviews)

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Human/Primate IL-6 Antibody
By Anonymous on 03/17/2022
Application: IHC Sample Tested: Colon tissue Species: Human
Immunohistochemistry Human/Primate IL-6 Antibody MAB206
Human/Primate IL-6 Antibody
By Anonymous on 08/02/2021
Application: IF Sample Tested: Brain (dorsal root ganglia) Species: Human
Immunofluorescence Human/Primate IL-6 Antibody MAB206
Human/Primate IL-6 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human/Primate IL-6 Antibody MAB206
Human/Primate IL-6 MAb (Clone 6708)
By Anonymous on 05/27/2019
Application: B/N Sample Tested: Breast cancer cells,Serum-free Cell Culture Media Species: Human
Human/Primate IL-6 Antibody
By Balaji Mahender on 12/19/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human
Human/Primate IL-6 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human/Primate IL-6 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human/Primate IL-6 Antibody
By Zoia Levashova on 11/01/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC). Paired with goat PC IL-6 antibody (AF-206-NA) as a detection antibody. A standard curve with recombinant human IL-6 (Cat# 206-IL/CF) is shown (1.6-25,000 pg/ml).

Other Human/Primate IL-6 Antibody MAB206
Human/Primate IL-6 Antibody
By Danlee Enzler on 04/24/2018
Application: IHC Sample Tested: Spleen tissue Species: Human
Human/Primate IL-6 Antibody
By Leslie Priddy on 04/13/2018
Application: WB Sample Tested: Tumor cell lyastes Species: Human
Human/Primate IL-6 Antibody
By Anonymous on 03/30/2018
Application: ICC/IF Sample Tested: Human affected ileum tissue from Crohn's patients Species: Human ileum tissue and Human normal resection margin tissue from Crohn's patients

Li C, Iness A, Yoon J, Grider JR, Murthy KS, Kellum JM, Kuemmerle JF. Increased autocrine IL-6 and STAT3 (Ser727) phosphorylation is profibrotic regulating TGF-β1 and Collagen I in muscle of stricturing Crohn's disease. J Immunol. 194(7):3422-31; 2015

Immunocytochemistry/Immunofluorescence Human/Primate IL-6 Antibody MAB206
Human/Primate IL‑6 Antibody
By Anonymous on 03/23/2018
Application: WB Sample Tested: Human recombinant test antibody Species: Human
Human/Primate IL-6 Antibody
By Qing Zhang on 05/16/2017
Application: Func Sample Tested: Kidney cancer cells Species: Human
Human/Primate IL-6 Antibody
By Michelle Chen on 04/26/2017
Application: B/N Sample Tested: Neutrophils Species: Human

IL-6 secretion was neutralized in neutrophils and its effect on tumor cell extravasation was assessed.

Block/Neutralize Human/Primate IL-6 Antibody MAB206