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Human/Primate MMP-3 Antibody

Catalog #: AF513
8 Citations 1 Review Datasheet / COA / SDS
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AF513
AF513-SP
Detection of Human MMP‑3 by Western Blot.
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Product Details
Citations (8)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate MMP-3 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate MMP-3 in ELISAs and Western blots. In sandwich ELISAs, less than 2.5% cross-reactivity with recombinant human
(rh) MMP‑10 is observed and less than 0.1% cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12, -13, and recombinant mouse MMP‑9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑3
Tyr18-Cys477 (Lys45Glu)
Accession # P08254
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑3 (Catalog # 513-MP), see our available Western blot detection antibodies

Human/Primate MMP-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate MMP‑3 Biotinylated Antibody (Catalog # BAF513)

Standard: Recombinant Human MMP-3 Protein, CF (Catalog # 513-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MMP-3 antibody by Western Blot. View Larger

Detection of Human MMP‑3 by Western Blot. Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (Catalog # WBC015) and lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MMP-3 antibody in Human Bladder Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑3 in Human Bladder Cancer Tissue. MMP-3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human/ Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MMP-3 antibody in Human Lung Cancer by Immunohistochemistry (IHC-P). View Larger

MMP‑3 in Human Lung Cancer. MMP-3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-3

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1) can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9, and pro-MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells, and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Long Name
Matrix Metalloproteinase 3
Entrez Gene IDs
4314 (Human); 17392 (Mouse)
Alternate Names
CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1

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Citations for Human/Primate MMP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Endosulfan promotes cell proliferation and extracellular matrix accumulation through TGF-beta/Smad signaling pathway in HRMCs
    Authors: S Liu, Y Hu, Y Wang, Y Sun, SL Qin, D Xu
    Ecotoxicology and environmental safety, 2021-11-30;228(0):113040.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. IL-3 Decreases Cartilage Degeneration by Downregulating Matrix Metalloproteinases and Reduces Joint Destruction in Osteoarthritic Mice
    J Immunol, 2016-05-13;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
    Authors: Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby
    PLoS ONE, 2016-03-31;0(0):1932-6203.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  5. Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
    Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
    Life Sci., 2008-04-20;82(23):1203-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
    Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
    FEBS Lett., 2007-04-09;581(9):1928-32.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007-01-29;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006-03-06;130(6):1754-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Primate MMP-3 Antibody

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Human/Primate MMP-3 Antibody
By Anonymous on 11/09/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

An ELISA was built using this antibody as both the capture and detection molecule. MMP-3 in human serum samples was measured using this assay.

ELISA Human/Primate MMP-3 Antibody AF513