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Human/Primate VEGF Antibody

Catalog #: MAB293
62 Citations 8 Reviews Datasheet / COA / SDS
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MAB293-SP
MAB293-100
MAB293-500
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Detection of Recombinant Human VEGF by Western Blot.
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Citations (62)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate VEGF Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and recombinant rat (rr)VEGF and no cross-reactivity with recombinant human (rh) VEGF-D is obseved.
Source
Monoclonal Mouse IgG2B Clone # 26503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
Ala27-Arg191
Accession # NP_001165097.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Human/Primate VEGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate VEGF165 Biotinylated Antibody (Catalog # BAF293)

Standard: Recombinant Human VEGF 165 Protein (Catalog # 293-VE)

Neutralization
Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 10 ng/mL Recombinant Human VEGF165.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human VEGF antibody by Western Blot. View Larger

Detection of Recombinant Human VEGF by Western Blot. Western blot shows 25 ng of Recombinant Human VEGF165(Catalog # 293-VE), Recombinant Human VEGF111(Catalog # 5336-VE), Recombinant Human VEGF121, aa 207-327 (Catalog # 4644-VS), Recombinant Human VEGF145(aa 27-171) (Catalog # 7626-VE), Recombinant Human VEGF162(Catalog # 2347-VE), Recombinant Human VEGF165b(Catalog # 3045-VE), Recombinant Human VEGF189(aa 27-215) (Catalog # 8147-VE), Recombinant Human VEGF165Extended Isoform (Catalog # 9018-VE), Recombinant Human VEGF-B167(Catalog # 751-VE), Recombinant Mouse VEGF164(Catalog # 493-MV), and Recombinant Rat VEGF164(Catalog # 564-RV). PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VEGF at approximately 15-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization Cell Proliferation Induced by VEGF<sub>165</sub>and Neutralization by Human VEGF Antibody. View Larger

Cell Proliferation Induced by VEGF165and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165(Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165(10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293). The ND50 is typically 10-60 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Citations for Human/Primate VEGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

62 Citations: Showing 1 - 10
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  1. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Effects of Human Deciduous Dental Pulp-Derived Mesenchymal Stem Cell-Derived Conditioned Medium on the Metabolism of HUVECs, Osteoblasts, and BMSCs
    Authors: R Kunimatsu, T Hiraki, K Rikitake, K Nakajima, NAR Putranti, T Abe, K Ando, A Nakatani, S Sakata, K Tanimoto
    Cells, 2022-10-14;11(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development, Neutralization
  3. Inhibition of the VEGF signaling pathway attenuates tumor?associated macrophage activity in liver cancer
    Authors: S Okikawa, Y Morine, Y Saito, S Yamada, K Tokuda, H Teraoku, K Miyazaki, S Yamashita, T Ikemoto, S Imura, M Shimada
    Oncology reports, 2022-02-16;47(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Tissue Inhibitor of Metalloproteinase-3 Ameliorates Diabetes-Induced Retinal Inflammation
    Authors: AM Abu El-Asr, A Ahmad, MI Nawaz, MM Siddiquei, A De Zutter, L Vanbrabant, PW Gikandi, G Opdenakker, S Struyf
    Frontiers in Physiology, 2022-01-10;12(0):807747.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy
    Authors: AM Abu El-Asr, MI Nawaz, A Ahmad, MM Siddiquei, E Allegaert, PW Gikandi, G De Hertogh, G Opdenakker
    Investigative Ophthalmology & Visual Science, 2021-07-01;62(9):32.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration
    Authors: KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh
    Cell Stem Cell, 2021-03-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Chrysin Inhibits High Glucose-Induced Migration on Chorioretinal Endothelial Cells via VEGF and VEGFR Down-Regulation
    Authors: ZY Liao, IC Liang, HJ Li, CC Wu, HM Lo, DC Chang, CF Hung
    Int J Mol Sci, 2020-08-02;21(15):.
    Species: Primate, Rhesus Macaque
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Snail promotes the generation of vascular endothelium by breast cancer cells
    Authors: Z Chang, Y Zhang, J Liu, Y Zheng, H Li, Y Kong, P Li, H Peng, Y Shi, B Cao, F Ran, Y Chen, Y Song, Q Ye, L Ding
    Cell Death Dis, 2020-06-15;11(6):457.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Neutralization
  9. Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth
    Authors: HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um
    Cells, 2020-05-28;9(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Quercetin attenuates adipose hypertrophy, in part through activation of adipogenesis in rats fed a high-fat diet
    Authors: DJ Perdicaro, C Rodriguez, J Gambarte T, RM Miatello, PI Oteiza, MA Vazquez Pr
    J. Nutr. Biochem., 2020-02-04;79(0):108352.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries
    Authors: AF Stassi, F Gasser, MML Velázquez, EM Belotti, NC Gareis, F Rey, HH Ortega, NR Salvetti, ME Baravalle
    Theriogenology, 2019-07-03;138(0):52-65.
    Species: Bovine, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates, Whole Tissue
    Applications: IHC, IHC-P, Western Blot
  12. TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
    Authors: P Zizza, R Dinami, M Porru, C Cingolani, E Salvati, A Rizzo, C D'Angelo, E Petti, CA Amoreo, M Mottolese, I Sperduti, A Chambery, R Russo, P Ostano, G Chiorino, G Blandino, A Sacconi, J Cherfils-V, C Leonetti, E Gilson, A Biroccio
    Nucleic Acids Res., 2019-04-23;0(0):.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  13. TSP-1 is downregulated and inversely correlates with miR-449c expression in Cushing's disease
    Authors: J Ren, C Gu, Y Yang, J Xue, Y Sun, F Jian, D Chen, L Bian, Q Sun
    J. Cell. Mol. Med., 2019-04-23;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells
    Authors: HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe
    Stem Cells, 2018-10-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Soluble E-cadherin promotes tumor angiogenesis and localizes to exosome surface
    Authors: MKS Tang, PYK Yue, PP Ip, RL Huang, HC Lai, ANY Cheung, KY Tse, HYS Ngan, AST Wong
    Nat Commun, 2018-06-11;9(1):2270.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Grape pomace extract induced beige cells in white adipose tissue from rats and in 3T3-L1 adipocytes
    Authors: C Rodriguez, DJ Perdicaro, MS Landa, A Fontana, A Antoniolli, RM Miatello, PI Oteiza, MA Vazquez Pr
    J. Nutr. Biochem., 2018-03-10;56(0):224-233.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin� protein MP0250: a preclinical study
    Authors: L Rao, K De Veirman, D Giannico, I Saltarella, V Desantis, MA Frassanito, AG Solimando, D Ribatti, M Prete, A Harstrick, U Fiedler, H De Raeve, V Racanelli, K Vanderkerk, A Vacca
    Oncotarget, 2018-01-30;9(17):13366-13381.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Investigating Colorimetric Protein Array Assay Schemes for Detection of Recurrence of Bladder Cancer
    Authors: S Gogalic, U Sauer, S Doppler, C Preininger
    Biosensors (Basel), 2018-01-24;8(1):.
    Species: Human
    Sample Types: Urine
    Applications: Array Development
  19. Application of an acellular dermal matrix to a rabbit model of oral mucosal defects
    Authors: X Xu, N Cui, E Wang
    Exp Ther Med, 2018-01-05;15(3):2450-2456.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Predictive Value of Clinical Findings and Plasma Biomarkers after Fourteen Days of Prednisone Treatment for Acute Graft-versus-host Disease.
    Authors: McDonald G, Tabellini L, Storer B, Martin P, Lawler R, Rosinski S, Schoch H, Hansen J
    Biol Blood Marrow Transplant, 2017-05-03;23(8):1257-1263.
    Species: Human
    Sample Types: Plasma
  21. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  22. Vessel morphometric parameters-correlation with histologic grade and VEGF expression in oligodendroglioma
    Authors: LB Strickland, S Brem, AM Rojiani, MV Rojiani
    Am J Cancer Res, 2017-04-01;7(4):973-981.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells
    Authors: W Katagiri, T Kawai, M Osugi, Y Sugimura-W, K Sakaguchi, T Kojima, T Kobayashi
    Maxillofac Plast Reconstr Surg, 2017-03-25;39(1):8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel�, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product
    Authors: C Thej, B Ramadasse, A Walvekar, AS Majumdar, S Balasubram
    Stem Cell Res Ther, 2017-02-28;8(1):47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms
    Authors: Lopa Leach
    Placenta, 2016-10-21;48(0):87-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  26. The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer
    Authors: Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang
    PLoS ONE, 2016-04-14;11(4):e0152789.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. VEGF-A acts via neuropilin-1 to enhance epidermal cancer stem cell survival and formation of aggressive and highly vascularized tumors
    Authors: D Grun, G Adhikary, RL Eckert
    Oncogene, 2016-01-25;35(33):4379-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
    Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
    J Biol Chem, 2015-07-24;290(36):22212-24.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke.
    Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H
    PLoS ONE, 2015-04-17;10(4):e0124362.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  30. Survivin expression promotes VEGF-induced tumor angiogenesis via PI3K/Akt enhanced beta-catenin/Tcf-Lef dependent transcription.
    Authors: Fernandez J, Rodriguez D, Valenzuela M, Calderon C, Urzua U, Munroe D, Rosas C, Lemus D, Diaz N, Wright M, Leyton L, Tapia J, Quest A
    Mol Cancer, 2014-09-09;13(0):209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014-09-02;5(0):4715.
    Species: Human
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  32. Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study.
    Authors: Aderhold C, Grobschmidt G, Sauter A, Faber A, Hormann K, Schultz J
    Oncol Lett, 2014-07-04;8(3):1112-1118.
    Species: Human
    Sample Types: Serum
    Applications: Bioassay
  33. Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
    Authors: Etulain J, Negrotto S, Tribulatti M, Croci D, Carabelli J, Campetella O, Rabinovich G, Schattner M
    PLoS ONE, 2014-04-30;9(4):e96402.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. Enhanced efficacy of combination therapy with adenoassociated virus-delivered pigment epithelium-derived factor and cisplatin in a mouse model of Lewis lung carcinoma.
    Authors: He S, Wu Q, Gong C, Luo S, Zhang S, Li M, Lu L, Wei Y, Yang L
    Mol Med Rep, 2014-04-04;9(6):2069-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  35. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013-12-31;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma.
    Authors: Townsend, Katelin, Spowart, Jaeline, Huwait, Hassan, Eshragh, Sima, West, Nathan R, Elrick, Mary A, Kalloger, Steve E, Anglesio, Michael, Watson, Peter H, Huntsman, David G, Lum, Julian J
    PLoS ONE, 2013-12-23;8(12):e82406.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Heat shock protein 27 attenuates neointima formation and accelerates reendothelialization after arterial injury and stent implantation: importance of vascular endothelial growth factor up-regulation.
    Authors: Ma X, Hibbert B, McNulty M, Hu T, Zhao X, Ramirez F, Simard T, de Belleroche J, O'Brien E
    FASEB J, 2013-10-18;28(2):594-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  38. High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha.
    Authors: Tan J, Prosser H, Vanags L, Monger S, Ng M, Bursill C
    FASEB J, 2013-09-10;28(1):206-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo.
    Authors: Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B
    Oncogene, 2010-07-19;29(39):5392-403.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  40. VEGF in the lung: a role for novel isoforms.
    Authors: Varet J, Varet, Julia, Douglas SK, Douglas, Samantha, Gilmartin L, Gilmartin, Laura, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B
    Am J Physiol Lung Cell Mol Physiol, 2010-03-12;298(6):L768-74.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  41. Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth.
    Authors: Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliner JD, McDonald DM
    Cancer Res., 2010-03-02;70(6):2213-23.
    Species: Human
    Sample Types: In Vivo
    Applications: Neutralization
  42. A small-molecule triptolide suppresses angiogenesis and invasion of human anaplastic thyroid carcinoma cells via down-regulation of the nuclear factor-kappa B pathway.
    Authors: Zhu W, Ou Y, Li Y, Xiao R, Shu M, Zhou Y, Xie J, He S, Qiu P, Yan G
    Mol. Pharmacol., 2009-01-21;75(4):812-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  44. FGF5 as an oncogenic factor in human glioblastoma multiforme: autocrine and paracrine activities.
    Authors: Allerstorfer S, Sonvilla G, Fischer H, Spiegl-Kreinecker S, Gauglhofer C, Setinek U, Czech T, Marosi C, Buchroithner J, Pichler J, Silye R, Mohr T, Holzmann K, Grasl-Kraupp B, Marian B, Grusch M, Fischer J, Micksche M, Berger W
    Oncogene, 2008-03-24;27(30):4180-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  45. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  46. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  47. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006-11-15;119(10):2313-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  48. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006-10-01;169(4):1167-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  49. Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
    Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
    Blood, 2006-09-21;109(1):122-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  50. Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice.
    Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K
    Cancer Res., 2006-07-01;66(13):6699-707.
    Species: Complex Species Category
    Sample Types: In Vivo
    Applications: Neutralization
  51. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
    Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
    Clin. Sci., 2006-05-01;110(5):575-85.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  52. Increased vascular endothelial growth factor and receptors: relationship to angiogenesis in asthma.
    Authors: Feltis BN, Wignarajah D, Zheng L, Ward C, Reid D, Harding R, Walters EH
    Am. J. Respir. Crit. Care Med., 2006-03-09;173(11):1201-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  53. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  54. Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
    Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
    Am. J. Physiol. Heart Circ. Physiol., 2005-10-07;290(3):H978-84.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  55. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
    Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
    Cancer Res., 2005-08-15;65(16):7241-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  56. Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo.
    Authors: Li L, Lin X, Staver M, Shoemaker A, Semizarov D, Fesik SW, Shen Y
    Cancer Res., 2005-08-15;65(16):7249-58.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  57. Possible mechanisms inducing glomerulations in interstitial cystitis: relationship between endoscopic findings and expression of angiogenic growth factors.
    Authors: Tamaki M, Saito R, Ogawa O, Yoshimura N, Ueda T
    J. Urol., 2004-09-01;172(3):945-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  59. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
    Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
    Cancer Res., 2003-02-15;63(4):887-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  60. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
    Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
    2002-04-01;160(4):1405-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  61. Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts.
    Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M
    Transplant. Proc., 2000-08-01;32(5):987-9.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  62. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
    Authors: Gu JW, Adair TH
    Am. J. Physiol., 1997-08-01;273(2):H628-33.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization

FAQs

  1. What are the differences between MAB293 and MAB293R?

    • MAB293 is produced by culturing a hybridoma cell line, whereas MAB293R is produced via recombinant DNA technology, based on the known, proprietary sequence of MAB293.  The benefit of recombinant antibodies is the ability to offer a more consistent antibody, with an indefinite supply.  As far as research applications, both products will work the same.

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Human/Primate VEGF Antibody
By Anonymous on 02/14/2022
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human
Western Blot Human/Primate VEGF Antibody MAB293
Human/Primate VEGF Antibody
By Anonymous on 08/09/2021
Application: WB Sample Tested: Metastatic breast cancer Species: Human
Western Blot Human/Primate VEGF Antibody MAB293
Human/Primate VEGF Antibody
By Karina Kinghorn on 07/16/2021
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

IF of HUVEC cells with exogenous VEGF internalized (red) and colocalizing with VEGF receptor (green)

Immunocytochemistry/Immunofluorescence Human/Primate VEGF Antibody MAB293
Human/Primate VEGF Antibody
By Anonymous on 05/27/2019
Application: B/N Sample Tested: Breast cancer cells,Serum-free Cell Culture Media Species: Human
Human/Primate VEGF Antibody
By Anonymous on 03/13/2019
Application: B/N Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

We used this VEGF-A neutralizing antibody (10 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize VEGF-A present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II CM (+/- VEGF-A Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay.
Cancer Res; 78(5) March 1, 2018 (Fig 6F)

Block/Neutralize Human/Primate VEGF Antibody MAB293
Human/Primate VEGF Antibody
By Zoia Levashova on 06/22/2018
Application: MSD &#40;Meso Scale Discovery&#41; assay Sample Tested: Aqueous humor,Retina/choroid,Vitreous humor Species: Porcine

After biotinylation, used as a capture reagent in MSD assay to detect ocular VEGF level in a swine model of laser-induced choroidal neovascularization. A standard curve with pig VEGF from Bio-Rad (Cat# PPP030) is shown (2-8,000 pg/ml).

Other Human/Primate VEGF Antibody MAB293
Human/Primate VEGF Antibody
By Alisha Freeman on 11/04/2017
Application: ELISA Sample Tested: CHO Chinese hamster ovary cell line Species: Hamster
Human/Primate VEGF Antibody
By Anonymous on 08/18/2017
Application: ELISA Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human
ELISA Human/Primate VEGF Antibody MAB293