Human PTPN13/PTPL1 Antibody

Catalog #: AF3577 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3577
AF3577-SP

Detection of Human PTPN13/PTPL1 by Western Blot.

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All PTPN13/PTPL1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTPN13/PTPL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human PTPN13/PTPL1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PTPN13/PTPL1
Met1-Arg500
Accession # Q12923

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PTPN13/PTPL1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN13/PTPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3577) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTPN13/PTPL1 at approximately 260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTPN13/PTPL1

Protein Tyrosine Phosphatase, Non-receptor Type 13, also called PTPN13, PTPL1, FAP-1, hPTP1E and PTP-BAS, is a 260 kDa intracellular protein that removes phosphates from tyrosine residues. Genetic aberrations in PTPN13 have been reported in a wide variety of cancers, including, bone, colon, hepatocellular, and germ cell line. Elevated levels of PTPN13 in cancer cell lines have also been correlated with resistance to Fas-induced apoptosis, possibly due to prevention of CD95 reaching the cell surface.

Long Name

Protein Tyrosine Phosphatase, Non-receptor Type 13

Entrez Gene IDs

5783 (Human); 19249 (Mouse); 498331 (Rat)

Alternate Names

EAP-1; FAP-1; hPTPE1; PNP1; protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associatedphosphatase); protein-tyrosine phosphatase 1, Fas-associated; PTP1E; PTP1EDKFZp686J1497; PTP-BAS; PTP-BASEC 3.1.3.48; PTP-BL; PTP-E1; PTPL1; PTPL1nonreceptor type 13; PTPLE; PTPN13; tyrosine-protein phosphatase non-receptor type 13