Human Purine Nucleoside Phosphorylase/PNP Antibody

Catalog #: MAB6486 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6486
MAB6486-SP

Detection of Human Purine Nucleoside Phosphorylase/PNP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Purine Nucleoside Phosphorylase/PNP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Purine Nucleoside Phosphorylase/PNP in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 773511

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Purine Nucleoside Phosphorylase/PNP
Met1-Ser289
Accession # P00491

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Purine Nucleoside Phosphorylase/PNP by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, Daudi human Burkitt's lymphoma cell line, HT1080 human fibrosarcoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Purine Nucleoside Phosphorylase/PNP Monoclonal Antibody (Catalog # MAB6486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Purine Nucleoside Phosphorylase/PNP at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Purine Nucleoside Phosphorylase/PNP in Human Liver. Purine Nucleoside Phosphorylase/PNP was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Purine Nucleoside Phosphorylase/PNP Monoclonal Antibody (Catalog # MAB6486) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Purine Nucleoside Phosphorylase/PNP

Purine Nucleoside Phosphorylase (PNP) catalyzes the phophorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. Physiological substrates of PNP include inosine, guanosine, and 2'-deoxyguanosine, but not adenosine (1). PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T‑lymphocyte function and neurologic dysfunction (2, 3). PNP is used in assays for the measurement of inorganic phosphate (4).

References

Schramm, V.L. (1998) Annu. Rev. Biochem. 67:693.
Stoop, W. et al. (1977) N. Eng. J. Med. 296:651.
Markert, M.L. (1991) Immunodefic. Rev. 3:45.
Webb, M.R. (1992) Proc. Natl. Acad. Sci. USA. 89:4884.

Entrez Gene IDs

4860 (Human)

Alternate Names

EC 2.4.2.1; FLJ94043; FLJ97288; Inosine phosphorylase; MGC117396; MGC125915; MGC125916; NPFLJ97312; nucleoside phosphorylase; PNP; PRO1837; PUNP; purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase