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Human PXR/NR1I2 Antibody

Catalog #: PP-H4417-00
1 Citation Datasheet / COA / SDS
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PP-H4417-00
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Citations (1)
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Human PXR/NR1I2 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human PXR1 and human PXR2. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H4417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human PXR/NR1I2
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions and 3 μg/mL under non-reducing conditions with the appropriatesecondary reagents to detect human PXR.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 2 μg/mL with the appropriatesecondary reagents to detect human PXR.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when stored at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PXR/NR1I2

Pregnane X Receptor (PXR; also known as SXR and NR1I2) is a 50 kDa member of the Nuclear Receptor (NR1) subfamily of the Nuclear Hormone Receptor family. It is a product of the PXR gene that gives rise to two basic isoforms; a 434 amino acid (aa) PXR1 form and 473 aa PXR2 form. PXR2 differs from PXR1 in that PXR2 contains a 39 aa N-terminal extension. Six PXR1 isoforms have been reported (Swiss Protein Accession # 075469). The anti-human PXR antibody recognizes all isoforms except the "B" isoforms which show a deletion of amino acids 1 - 55 (MEVRP...FNVM). PXR forms a heterodimer with Retinoid X Receptor (RXR) and binds pregnanolone.

Long Name
Pregnane X Receptor
Entrez Gene IDs
8856 (Human); 18171 (Mouse)
Alternate Names
BXR; NR1I2; nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group I, member 2; ONR1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR; PAR1; PAR2; Pregnane X receptor; PRR; PXR; PXRPARq; SAR; Steroid and xenobiotic receptor; SXR; SXRBXR

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Citation for Human PXR/NR1I2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Pregnane X receptor suppresses proliferation and tumourigenicity of colon cancer cells.
    Authors: Ouyang N, Ke S, Eagleton N
    Br. J. Cancer, 2010-06-08;102(12):1753-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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