Human Rad17 Antibody

Catalog #: MAB1926 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1926
MAB1926-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Rad17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rad17 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 223802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rad17
Asn2-Arg240
Accession # NP_579922

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunoprecipitation

2-3 µg/500 µg cell lysate

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human Rad17. Rad17 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2-3 µg Mouse Anti-Human Rad17 Monoclonal Antibody (Catalog # MAB1926) for 1 hour on ice. Rad17-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Rad17 (left panel) was detected by Western blot using 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad17

The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).

Entrez Gene IDs

5884 (Human); 310034 (Rat)

Alternate Names

CCYC; cell cycle checkpoint protein (RAD17); cell cycle checkpoint protein RAD17; FLJ41520; HRAD17; R24L; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17 homolog (S. pombe); Rad17; Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog