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Human/Rat CCR4 PerCP-conjugated Antibody

Catalog #: FAB1567C
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1567C-025
FAB1567C-100
Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat CCR4 PerCP-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Stains human CCR4-transfected cell lines but not the corresponding parent cell lines. Also detects human CCR4 on human PBLs and platelets and rat CCR4 on rat splenocytes.
Source
Monoclonal Mouse IgG2B Clone # 205410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CCR4 transfectants
Met1-Leu360
Accession # P51679
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Mouse Anti-Human/Rat CCR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1567C) or (B) Mouse IgG2BPerCP Isotype Control (Catalog # IC0041C). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR4 antibody in Rat Peripheral Blood Cells antibody by Flow Cytometry. View Larger

Detection of CCR4 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Mouse Anti-Human/Rat CCR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1567C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR4

CCR4 is a G protein-linked seven transmembrane domain chemokine receptor that binds the chemokines CCL17/TARC and CCL22/MDC. Current evidence suggests that CCR4 expression is associated with Th-2 type T cells and with platelets. CCR4 expression has also been reported in mature dendritic cells.

Entrez Gene IDs
1233 (Human); 12773 (Mouse); 171054 (Rat); 403541 (Canine); 102120178 (Cynomolgus Monkey)
Alternate Names
C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5

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Citation for Human/Rat CCR4 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes
    Authors: T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart
    Mol Metab, 2017-09-27;6(11):1529-1539.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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