Human/Rat CCR8 APC-conjugated Antibody
Human/Rat CCR8 APC-conjugated Antibody Summary
Met1-Leu355
Accession # P51685
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CCR8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 APC-conjugated Monoclonal Antibody (Catalog # FAB1429A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR8
CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.
Product Datasheets
Citations for Human/Rat CCR8 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer
Authors: S Su, J Liao, J Liu, D Huang, C He, F Chen, L Yang, W Wu, J Chen, L Lin, Y Zeng, N Ouyang, X Cui, H Yao, F Su, JD Huang, J Lieberman, Q Liu, E Song
Cell Res, 2017-03-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
J Immunol, 2014-02-19;192(6):2634-42.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
Authors: Ahern D, Lloyd CM, Robinson DS
Allergy, 2009-02-07;64(8):1121-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
J. Immunol., 2007-04-01;178(7):4184-93.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
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Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?
The staining with this antibody is temperature dependent; it will stain at 4 C and room temperature, however, staining is stronger at 37 C.
Reviews for Human/Rat CCR8 APC-conjugated Antibody
Average Rating: 4.7 (Based on 3 Reviews)
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Band is is clear and beautiful
Gated on monocyte FSC-A and SSC-A population. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
