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Human/Rat Fibronectin Antibody

Catalog #: AF1918
11 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1918
AF1918-SP
Detection of Human Fibronectin by Western Blot.
4 Images
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Fibronectin Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human Fibronectin in direct ELISAs. Detects human and rat Fibronectin in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Human plasma-derived Fibronectin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line
Simple Western
10 µg/mL
HepG2 human hepatocellular carcinoma cell line
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Fibronectin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry Fibronectin antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

Fibronectin in A549 Human Cell Line. Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Fibronectin antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Fibronectin in Human Liver. Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human Fibronectin by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016).The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple WesternTM

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin

Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.

Entrez Gene IDs
2335 (Human); 280794 (Bovine)
Alternate Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

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Citations for Human/Rat Fibronectin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep
    Authors: D Michalski, W Reimann, E Spielvogel, B Mages, B Biedermann, H Barthel, B Nitzsche, S Schob, W Härtig
    International Journal of Molecular Sciences, 2022-05-24;23(11):.
    Species: Mouse, Rat, Sheep
    Sample Types: Whole Tissue
    Applications: IHC
  2. Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma
    Authors: J Xie, M Sun, D Zhang, C Chen, S Lin, G Zhang
    FEBS Open Bio, 2021-10-03;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation
    Authors: A Acheva, S Haghdoost, A Sollazzo, V Launonen, M Kämäräinen
    Oxid Med Cell Longev, 2019-09-08;2019(0):4120379.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastoma
    Authors: T van Gronin, N Akogul, EM Westerhout, A Chan, NE Hasselt, DA Zwijnenbur, M Broekmans, P Stroeken, F Haneveld, GKJ Hooijer, CD Savci-Heij, A Lakeman, R Volckmann, P van Sluis, LJ Valentijn, J Koster, R Versteeg, J van Nes
    Nat Commun, 2019-04-04;10(1):1530.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes
    Authors: AL Jolly, S Rau, AK Chadha, EA Abdulrahee, D Dean
    MBio, 2019-03-19;10(2):.
    Species: Human
    Sample Types: Whole Cells
  6. Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
    Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
    Frontiers in Bioengineering and Biotechnology, 2018-11-01;0(0):2296-4185.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Neuroblastoma is composed of two super-enhancer-associated differentiation states
    Authors: T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg
    Nat. Genet., 2017-06-26;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  8. Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET.
    Authors: Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P
    Clin Cancer Res, 2013-03-26;19(9):2381-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  9. PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
    J. Cell. Sci., 2012-02-17;125(0):2276-87.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Semen clusterin is a novel DC-SIGN ligand.
    Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
    J. Immunol., 2011-10-17;187(10):5299-309.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Western Blot
  11. Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
    Authors: Massam-Wu T, Chiu M, Choudhury R
    J. Cell. Sci., 2010-08-10;123(0):3006-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Rat Fibronectin Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human Fibronectin Antibody
By Anonymous on 10/01/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human

Paired with MAB1918

ELISA Human Fibronectin Antibody AF1918
Human Fibronectin Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human