Human/Rat Neuroligin 1/NLGN1 Antibody

Catalog #: AF4340 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4340
AF4340-SP

Detection of Human and Rat Neuroligin 1/ NLGN1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Neuroligin 1/NLGN1 Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects recombinant rat Neuroligin 1/NLGN1 in direct ELISAs. Detects human and rat Neuroligin 1/NLGN1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuroligin 1/NLGN1
Gln46-Leu696
Accession # Q62765

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat Neuroligin 1/ NLGN1 by Western Blot. Western blot shows lysates of rat embryonic cortical neurons, rat embryonic hippocampal neurons, and human brain (cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Rat Neuroligin 1/ NLGN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroligin 1/NLGN1 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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Neuroligin 1/NLGN1 in Rat Brain. Neuroligin 1/NLGN1 was detected in perfusion fixed frozen sections of rat brain (cortex) using Sheep Anti-Rat Neuroligin 1/ NLGN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies, processes, and dendritic spines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 1/NLGN1

Neuroligin 1 (neurexin ligand 1; also NL1) is a 116 kDa member of the type-B carboxyesterase/lipase family of proteins. It is postsynaptically expressed on neurons and initiates excitatory presynapse maturation through binding to a select isoform of beta -neurexin. Mature rat NL1 is 798 amino acids (aa) in length. It is expressed as a type I transmembrane glycoprotein that contains a 652 aa extracellular domain (ECD) (aa 46‑697) and a 125 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain (aa 54‑612). Rat NL1 has at least three alternate splice forms. One shows a deletion of aa 165‑184 plus aa 298‑306, while the remaining two show deletions of either aa 165‑184, or aa 298‑306, respectively. Rat ECD shares 97% and 93% aa identity with mouse and human NL1 CED, respectively.

Entrez Gene IDs

22871 (Human); 192167 (Mouse); 116647 (Rat)

Alternate Names

EC 3.1.1; EC 3.1.1.1; KIAA1070neuroligin-1; MGC45115; Neuroligin 1; NL1; Nlg; NLGN1