Human/Rat PKC beta 1 Antibody

Catalog #: AF3759 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3759
AF3759-SP

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All PKC beta 1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat PKC beta 1 Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat PKC beta 1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human PKCδ is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKC beta 1
Lys607-Val671
Accession # P05771

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human PKC beta 1
Recombinant Rat PKC beta 1

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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PKC beta 1 in Rat Cortical Stem Cells. PKC beta 1 was detected in immersion fixed rat cortical stem cells using Goat Anti-Human/Rat PKC beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC beta 1

PKC beta 1 is a 671 aa, 77 kDa member of the PKC subfamily of Ser/Thr family of protein kinases. PKC beta 1 is a Ca²⁺-activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC beta 2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC beta 1 are 100% identical.

Long Name

Protein Kinase C beta 1

Entrez Gene IDs

5579 (Human); 18751 (Mouse); 25023 (Rat)

Alternate Names

PKC beta 1; PKCB;PKCbeta;PKC-beta;PKCI(2);PRKCB;PRKCB1;PRKCB2;Protein kinase C beta type; PRKCB; PRKCB1; PRKCB2