Human/Rat Yes Antibody

Catalog #: AF3205 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3205
AF3205-SP

Detection of Human/Rat Yes by Western Blot.

2 Images

All Yes Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Yes Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat Yes.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Yes
Met1-Gly88
Accession # P07947.3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Rat Yes by Western Blot. Western blot shows lysates of L6 rat myoblast cell line, Rat-2 rat embryonic fibroblast cell line, MCF-7 human breast cancer cell line and, MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

View Larger

Yes in A431 Human Cell Line. Yes was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yes

Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1

Entrez Gene IDs

7525 (Human); 22612 (Mouse); 24884 (Rat)

Alternate Names

cellular yes-1 protein; c-yes; EC 2.7.10; EC 2.7.10.2; HsT441; P61-YES; Proto-oncogene c-Yes; proto-oncogene tyrosine-protein kinase Yes; v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; Yamaguchi sarcoma oncogene; Yes; YES1