Human RENT1/UPF1 Antibody

Catalog #: AF5879 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5879
AF5879-SP

Detection of Human RENT1/UPF1 by Western Blot.

1 Image

All RENT1/UPF1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RENT1/UPF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RENT1/UPF1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RENT1/UPF1
Asp351-Arg600
Accession # NP_002902

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human RENT1/UPF1 by Western Blot. Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RENT1/UPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5879) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RENT1/UPF1 at approximately 125-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RENT1/UPF1

UPF1 (Up-frameshift mutation-1; also RENT1, SMG-2 and NORF1) is a 125-130 kDa member of the DNA2/NAM7 helicase family of molecules. It is ubiquitously expressed, and promotes the turnover of mRNA containing premature stop codons. UPF1 is a cytoplasmic factor that contributes to the formation of a UPF complex. During the translation of properly transcribed mRNA, unphosphorylated UPF1 is blocked from interacting with eRF3, a translation initiation factor. In the presence of an improper stop codon, a detection complex termed EJC initiates phosphorylation of UPF1, allowing it to bind to eRF3 and repress translation. Human UPF1 is 1129 amino acids (aa) in length. It contains one C2H2-type Zn finger motif (aa 131-159), an NTPase domain (aa 486-545) and three phosphorylation sites at Ser1107/1127/1129. There are two potential isoforms that show deletions of aa 54-58 and 353-363, respectively. Over aa 351-600, human UPF1 shares 99% aa identity with mouse UPF1.

Long Name

Regulator of Nonsense Transcripts 1

Entrez Gene IDs

5976 (Human); 19704 (Mouse)

Alternate Names

ATP-dependent helicase RENT1; EC 3.6.1; EC 3.6.4.-; FLJ43809; HUPF1; KIAA0221FLJ46894; Nonsense mRNA reducing factor 1; NORF1; NORF1delta helicase; pNORF1; regulator of nonsense transcripts 1; RENT1; RENT1UP Frameshift 1; UPF1 regulator of nonsense transcripts homolog (yeast); UPF1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; yeast Upf1p homolog