Human ROBO2 Antibody Summary
Ser22-Pro859
Accession # Q9HCK4.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROBO2 in HEK293 Human Cell Line transfected with Human ROBO2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ROBO2 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-Human ROBO2 monoclonal antibody (Catalog # MAB3147) followed by Allophycocyanin-conjugated anti-Mouse IgG secondary antibody (F0101B). Quadrant markers were set based on isotype control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.
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ROBO2 in Human Brain (Cortex). ROBO2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ROBO2 Monoclonal Antibody (Catalog # MAB3147) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROBO2
Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1‑3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31‑504) and three fibronectin (FN) type III domains (aa 522‑826). The cytoplasmic region contains multiple 15‑20 aa long CC (conserved cytoplasmic) motifs (C0‑C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186‑1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22‑285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD shares 98% aa sequence identity with the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1‑ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).
- Rajagopalan, S. et al. (2000) Neuron 28:767.
- Guthrie, S. (2004) Curr. Biol. 14:R632.
- Guthrie, S. (2001) Curr. Biol. 11:R300.
- Seeger, M. et al. (1993) Neuron 10:409.
- Kidd, T. et al. (1998) Cell 92:205.
- Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
- Bashaw, G.J. et al. (2000) Cell 101:703.
- Jen, J.C. et al. (2004) Science 304:1509.
- GenBank Accession # Q9KCK4.
- Isogai, T. et al. (2002) GenBank Accession # AK074780.
- Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
- Long, H. et al. (2004) Neuron 42:213.
- Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534.
- Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.
Product Datasheets
Citations for Human ROBO2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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SLIT2/ROBO2 signaling pathway inhibits nonmuscle myosin IIA activity and destabilizes kidney podocyte adhesion
JCI Insight, 2016-11-17;1(19):e86934.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
COUP-TFII regulates satellite cell function and muscular dystrophy
J Clin Invest, 2016-09-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
Exp. Cell Res., 2011-12-14;318(4):371-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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