Human ROBO4 Antibody Summary
Gln28-Arg467
Accession # Q8WZ75
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROBO4
ROBO4, also called magic roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family (1). ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration (2). Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium (1, 2). Expression in early hematopoietic progenitors is also reported (3). The human ROBO4 cDNA encodes 1012 amino acids (aa), including a 27 aa signal sequence, a 440 aa extracellular domain (ECD) containing two C2‑type Ig domains and two fibronectin type III (FNIII) domains, a transmembrane domain and an intracellular domain. ROBO4 diverges from other ROBOs in the number of Ig, FNIII and cytoplasmic CC domains (1, 4). Within the ECD, human ROBO4 shares 80%, 80%, 87% and 88% aa identity with mouse, rat, bovine and canine ROBO4, respectively. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis (1, 2, 4 ‑ 6). It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF‑induced vascular leak (6 ‑ 9). ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers (2, 4 ‑ 7, 9 ‑ 13). ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation (2, 4 ‑ 13). Effects on cell movement may be mediated through intracellular binding of WASP‑, Ras/Rac/Rho‑, Mena‑, Src‑ or Paxillin‑related proteins, all of which affect the cytoskeleton (5 ‑ 7, 10 ‑ 12). Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis (13).
- Huminiecki, L. et al. (2002) Genomics 79:547.
- Legg, J.A. et al. (2008) Angiogenesis 11:13.
- Shibata, F. et al. (2009) Stem Cells 27:183.
- Park, K.W. et al. (2003) Dev. Biol. 261:251.
- Seth, P. et al. (2005) Biochem. Biophys. Res. Commun. 332:533.
- Jones, C.A. et al. (2008) Nat. Med. 14:448.
- Jones, C.A. et al. (2009) Nat. Cell Biol. 11:1325.
- Chen, H. et al. (2010) Adv. Exp. Med. Biol. 664:457.
- London, N.R. et al. (2009) J. Thromb. Haemost. 7:57.
- Verissimo, A.R. et al. (2009) Biochem. Soc. Trans. 37:1214.
- Sheldon, H. et al. (2009) FASEB J. 23:513.
- Kaur, S. et al. (2008) BMC Cell Biol. 9:61.
- Suchting, S. et al. (2005) FASEB J. 19:121.
Product Datasheets
Citations for Human ROBO4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis
Authors: W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang
Scientific Reports, 2022-03-14;12(1):4352.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
Nat Med, 2015-04-20;21(5):483-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
Exp. Cell Res., 2011-12-14;318(4):371-8.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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