Human ROBO4 Antibody

Catalog #: AF2366
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2366
AF2366-SP

All ROBO4 Antibodies

Product Details

Citations (1)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ROBO4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO2, and rhROBO3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROBO4
Gln28-Arg467
Accession # Q8WZ75

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ROBO4

Flow Cytometry

0.25 µg/10⁶ cells

HUVEC human umbilical vein endothelial cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO4

ROBO4 belongs to the ROBO family, which are molecular guidance receptors that interact with Slit ligand to regulate axon guidance, neuronal migration and leukocyte chemotaxis. The expression of ROBO4 is restricted in the endothelium, notably in areas of angiogenesis.

Long Name

Roundabout Homolog 4

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Alternate Names

ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; ROBO4; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ROBO4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Robo 4 - the double-edged sword in prostate cancer: impact on cancer cell aggressiveness and tumor vasculature
Authors: A Pircher, G Schäfer, A Eigentler, R Pichler, M Puhr, E Steiner, W Horninger, E Gunsilius, H Klocker, I Heidegger
Int J Med Sci, 2019-01-01;16(1):115-124.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

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