Human ROBO4 PE-conjugated Antibody Summary
Gln28-Arg467
Accession # Q8WZ75
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROBO4 in Human ROBO4 Transfectants by Flow Cytometry. Human ROBO4 transfectants were stained with Mouse Anti-Human ROBO4 PE-conjugated Monoclonal Antibody (Catalog # FAB2454P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROBO4
ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family (1). ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration (2). Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium (1, 2). Expression in early hematopoietic progenitors is also reported (3). The human ROBO4 cDNA encodes 1012 amino acids (aa), including a 27 aa signal sequence, a 440 aa extracellular domain (ECD) containing two C2-type Ig domains and two fibronectin type III (FNIII) domains, a transmembrane domain and an intracellular domain. ROBO4 diverges from other ROBOs in the number of Ig, FNIII and cytoplasmic CC domains (1, 4). Within the ECD, human ROBO4 shares 80%, 80%, 87% and 88% aa identity with mouse, rat, bovine and canine ROBO4, respectively. Vascular endothelial ROBO4 is expressed at highest levels during development and vascular remodeling, including tumor angiogenesis (1, 2, 4-6). It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF-induced vascular leak (6-9). ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers (2, 4-7, 9-13). ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation (2, 4-13). Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src- or Paxillin-related proteins, all of which affect the cytoskeleton (5-7, 10-12). Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis (13).
- Huminiecki, L. et al. (2002) Genomics 79:547.
- Legg, J.A. et al. (2008) Angiogenesis 11:13.
- Shibata, F. et al. (2009) Stem Cells 27:183.
- Park, K.W. et al. (2003) Dev. Biol. 261:251.
- Seth, P. et al. (2005) Biochem. Biophys. Res. Commun. 332:533.
- Jones, C.A. et al. (2008) Nat. Med. 14:448.
- Jones, C.A. et al. (2009) Nat. Cell Biol. 11:1325.
- Chen, H. et al. (2010) Adv. Exp. Med. Biol. 664:457.
- London, N.R. et al. (2009) J. Thromb. Haemost. 7:57.
- Verissimo, A.R. et al. (2009) Biochem. Soc. Trans. 37:1214.
- Sheldon, H. et al. (2009) FASEB J. 23:513.
- Kaur, S. et al. (2008) BMC Cell Biol. 9:61.
- Suchting, S. et al. (2005) FASEB J. 19:121.
Product Datasheets
Citation for Human ROBO4 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro.
Authors: Zhang Y, Fisher N, Newey SE
Stem Cells Dev., 2009-03-01;18(2):359-75.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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