Human S100A11 Antibody

Catalog #: MAB4874 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4874
MAB4874-SP

Detection of Human S100A11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100A11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human S100A11 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A7, A8, A9, A10, A13, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 672816

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human S100A11
Ala2-Thr105
Accession # P31949

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human S100A11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human S100A11 Monoclonal Antibody (Catalog # MAB4874) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A11

S100A11 (also S100C and calgizzarin) is a 10-12 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by smooth muscle and keratinocytes. Intracellularly, it suppresses growth; extracellularly, it exists as both a monomer, homodimer, and heterodimer with S100B, binds to RAGE, induces EGF, and promotes cell growth. Human S100A11 is 105 amino acids (aa) in length. It contains two EF-hand motifs (aa 13-49 and 55-90) and one high-affinity Ca-binding site (aa 68-79), and binds annexin I with its C-terminal half. There may be one alternative splice form that apparently contains a series of mutations over aa 61-79 and shows 90% overall aa identity to the standard form. Full-length human S100A11 shares 78% and 82% aa identity with mouse and porcine S100A11, respectively.

Long Name

S100 Calcium Binding Protein A11

Entrez Gene IDs

6282 (Human); 20195 (Mouse); 445415 (Rat)

Alternate Names

Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70; MLN70; protein S100-A11; Protein S100-C; S100 calcium binding protein A11; S100 calcium-binding protein A11 (calgizzarin); S100 calcium-binding protein A11; S100A11; S100C; S100CS100 calcium binding protein A11 (calgizzarin)