Human S100A8/S100A9 Heterodimer ELISA Kit - Quantikine

Name: Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit
Brand: R&D Systems
SKU: DS8900
Rating: 4.5 (2 reviews)

Catalog #: DS8900
15 Citations 2 Reviews Datasheet / COA / SDS

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DS8900

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QC223: Quantikine Immunoassay Control Set 1099 Human S100A8/S100A9

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Human S100A8/S100A9 Heterodimer ELISA Standard Curve

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Citations (15)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL), Human Milk (10 uL), Fecal Extract (50 uL)

Sensitivity

0.215 ng/mL

Assay Range

0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Specificity

Natural and recombinant human S100A8/S100A9 Heterodimer.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human S100A8/S100A9 Heterodimer Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human S100A8/S100A9 Heterodimer in cell culture supernates, tissue lysastes, serum, plasma, saliva, urine, human milk, and fecal extract. It contains E. coli-expressed recombinant human S100A8/S100A9 Heterodimer and antibodies raised against the recombinant factor. Results obtained using natural human S100A8/S100A9 Heterodimer showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human S100A8/S100A9 Heterodimer.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.64	12.1	25.3	6.09	12.6	24.6
Standard Deviation	0.155	0.37	1.14	0.352	0.606	0.792
CV%	2.7	3.1	4.5	5.8	4.8	3.2

Recovery

The recovery of human S100A8/S100A9 Heterodimer spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	85	79-96
Cell Lysis Buffer (n=3)	94	80-104
Human Milk (n=4)	96	83-103
Urine (n=4)	100	91-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human S100A8/S100A9 Heterodimer were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human S100A8/S100A9 Heterodimer ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8/S100A9 Heterodimer

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name:

S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9

Entrez Gene IDs:

6279 (Human)

Alternate Names:

Calprotectin; S100A8/S100A9 Heterodimer

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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MSC-Derived Exosomes Mitigate Myocardial Ischemia/Reperfusion Injury by Reducing Neutrophil Infiltration and the Formation of Neutrophil Extracellular Traps
Authors: Feng, Y;Bao, X;Zhao, J;Kang, L;Sun, X;Xu, B;
International journal of nanomedicine
Species: Human
Sample Types: Plasma
S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo
Authors: F Real, A Zhu, B Huang, A Belmellat, A Sennepin, T Vogl, C Ransy, M Revol, R Arrigucci, A Lombès, J Roth, ML Gennaro, F Bouillaud, S Cristofari, M Bomsel
Nature Communications, 2022-10-11;13(1):5956.
Species: Human
Sample Types: Tissue Homogenates
ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation
Authors: Z Li, O McGinn, Y Wu, A Bahreini, NM Priedigkei, K Ding, S Onkar, C Lampenfeld, CA Sartorius, L Miller, M Rosenzweig, O Cohen, N Wagle, JK Richer, WJ Muller, L Buluwela, S Ali, TC Bruno, DAA Vignali, Y Fang, L Zhu, GC Tseng, J Gertz, JM Atkinson, AV Lee, S Oesterreic
Nature Communications, 2022-04-19;13(1):2011.
Species: Human
Sample Types: Plasma
Core genes involved in the regulation of acute lung injury and their association with COVID-19 and tumor progression: A bioinformatics and experimental study
Authors: AV Sen'kova, IA Savin, EV Brenner, MA Zenkova, AV Markov
PLoS ONE, 2021-11-22;16(11):e0260450.
Species: Human
Sample Types: Plasma
An Intervention With Michigan-Grown Wheat in Healthy Adult Humans to Determine Effect on Gut Microbiota: Protocol for a Crossover Trial
Authors: GA Kinney, EN Haddad, LS Garrow, PKW Ng, SS Comstock
JMIR research protocols, 2021-10-06;10(10):e29046.
Species: Human
Sample Types: Stool
Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits
Authors: IA Drosatos, JN Tsoporis, S Izhar, S Gupta, G Tsirebolos, E Sakadakis, AS Triantafyl, A Rigopoulos, D Rigopoulos, LS Rallidis, I Rizos, TG Parker
Biomolecules, 2021-09-13;11(9):.
Species: Human
Sample Types: Plasma
Biomarkers of systemic inflammation, soluble IL-2Ralpha and the multiple sclerosis-associated IL2RA SNP rs2104286 in healthy subjects and multiple sclerosis patients
Authors: S Buhelt, HB Søndergaar, MR Mahler, S Cobanovic, L Börnsen, C Ammitzbøll, AB Oturai, F Sellebjerg
Multiple sclerosis and related disorders, 2021-07-07;54(0):103140.
Species: Human
Sample Types: Serum
Circulating levels of tight junction proteins in multiple sclerosis: Association with inflammation and disease activity before and after disease modifying therapy
Authors: A Olsson, S Gustavsen, AR Langkilde, TH Hansen, F Sellebjerg, H Bach Sønde, AB Oturai
Multiple sclerosis and related disorders, 2021-07-03;54(0):103136.
Species: Human
Sample Types: Serum
Biomarkers of inflammation and epithelial barrier function in multiple sclerosis
Authors: A Olsson, S Gustavsen, IC Hasselbalc, AR Langkilde, F Sellebjerg, AB Oturai, HB Søndergaar
Mult Scler Relat Disord, 2020-09-19;46(0):102520.
Species: Human
Sample Types: Serum
Toll-like receptor involvement in adolescent scoliotic facet joint degeneration
Authors: DG Bisson, K Sheng, S Kocabas, E Krock, A Teles, N Saran, JA Ouellet, L Haglund
J. Cell. Mol. Med., 2020-08-27;0(0):.
Species: Human
Sample Types: Cell Culture Supernates

Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
Authors: DR Shores, J Fundora, M Go, F Shakeel, S Brooks, SM Alaish, J Yang, CP Sodhi, DJ Hackam, A Everett
BMC Pediatr, 2020-05-26;20(1):250.
Species: Human
Sample Types: Serum

Body Mass Index and Calprotectin Blood Level Correlation in Healthy Children: An Individual Patient Data Meta-Analysis
Authors: A Grand, E Rochette, F Dutheil, D Gozal, V Calcaterra, R Berni Cana, N Cobanoglu, JPM Derikx, G Terrin, B Pereira, E Merlin
J Clin Med, 2020-03-20;9(3):.
Species: Human
Sample Types: Plasma

Activation of a TLR9 mediated innate immune response in preeclampsia
Authors: RD Williamson, FP McCarthy, LC Kenny, CM McCarthy
Sci Rep, 2019-04-11;9(1):5920.
Species: Human
Sample Types: Plasma

The rationale and design of the personal diet study, a randomized clinical trial evaluating a personalized approach to weight loss in individuals with pre-diabetes and early-stage type 2 diabetes
Authors: CJ Popp, DE St-Jules, L Hu, L Ganguzza, P Illiano, M Curran, H Li, A Schoenthal, M Bergman, AM Schmidt, E Segal, A Godneva, MA Sevick
Contemp Clin Trials, 2019-03-04;0(0):.
Species: Human
Sample Types:

Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection
Nat Med, 2016-09-26;0(0):.
Species: Human
Sample Types: Serum