Human S100P Antibody Summary
Met1-Lys95
Accession # P25815
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human S100P by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma parental cell line and S100P knockout HT-29 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100P at approximately 10 kDa (as indicated) in the parental HT-29 cell line, but is not detectable in knockout HT-29 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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S100P in Human Stomach. S100P was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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S100P Specificity is Shown by Immunocytochemistry in Knockout Cell Line. S100P was detected in immersion fixed HT-29 human colon adenocarcinoma cell line but is not detected in S100P knockout (KO) HT-29 Human Cell Line cell line using Goat Anti-Human S100P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2957) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100P
S100P is a member of the S100 protein family, whose members are calcium-binding proteins containing two characteristic E-F hand motifs. S100P proteins exist as homodimers or heterodimers (e.g. with S100A1). They interact with target proteins in a Ca++-dependent manner to regulate cell functions. S100P is localized intracellularly but can also be released from cells to act extracellularly. The amino acid sequence of human S100P is 47% identical to that of rat S100P.
Product Datasheets
Citations for Human S100P Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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An Extracellular/Membrane-Bound S100P Pool Regulates Motility and Invasion of Human Extravillous Trophoblast Lines and Primary Cells
Authors: Lancaster, T;Tabrizi, MEA;Repici, M;Gupta, J;Gross, SR;
Biomolecules
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
The Role of the C-Terminal Lysine of S100P in S100P-Induced Cell Migration and Metastasis
Authors: TM Ismail, SR Gross, T Lancaster, PS Rudland, R Barracloug
Biomolecules, 2021-10-06;11(10):.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Functional Assay -
An immunostaining panel of C-reactive protein, N-cadherin, and S100P is useful for intrahepatic cholangiocarcinoma subtyping
Authors: M Akita, R Sawada, M Komatsu, N Suleman, T Itoh, T Ajiki, N Heaton, T Fukumoto, Y Zen
Human pathology, 2020-12-13;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Morphological subclassification of intrahepatic cholangiocarcinoma: etiological, clinicopathological, and molecular features.
Authors: Liau J, Tsai J, Yuan R, Chang C, Lee H, Jeng Y
Mod Pathol, 2014-01-10;27(8):1163-73.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).
Authors: Shimamoto, Seiko, Kubota, Yasuo, Yamaguchi, Fuminori, Tokumitsu, Hiroshi, Kobayashi, Ryoji
J Biol Chem, 2013-01-23;288(10):7158-68.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma.
Authors: Bartling B, Rehbein G, Simm A, Silber RE, Hofmann HS
Int. J. Oncol., 2010-04-01;36(4):1015-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C.
Authors: Ludvigsen M, Jacobsen C, Maunsbach AB, Honore B
Proteomics, 2009-12-01;9(23):5267-87.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
S100P is a molecular determinant of E-cadherin function in gastric cancer
Authors: Patrícia Carneiro, Ana Margarida Moreira, Joana Figueiredo, Rita Barros, Patrícia Oliveira, Maria Sofia Fernandes et al.
Cell Communication and Signaling
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