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Human S1P1/EDG-1 Antibody

Catalog #: MAB2016
9 Citations Datasheet / COA / SDS
Clone 218713 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB2016-SP
MAB2016-500
MAB2016-100
Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry.
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Citations (9)
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Human S1P1/EDG-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S1P1/EDG‑1 by flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 218713
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P1/EDG‑1
Met1-Ser382
Accession # P21453
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<sub>1</sub>/EDG-1 antibody in CHO Chinese Hamster Cell Line Transfected with Human S1P<sub>1</sub>/EDG-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P1/EDG-1

S1P1, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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Citations for Human S1P1/EDG-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Sphingosine-1-phosphate receptor 1/5 selective agonist alleviates ocular vascular pathologies
    Authors: Nakamura, S;Yamamoto, R;Matsuda, T;Yasuda, H;Nishinaka, A;Takahashi, K;Inoue, Y;Kuromitsu, S;Shimazawa, M;Goto, M;Narumiya, S;Hara, H;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The GPCR adaptor protein Norbin regulates S1PR1 trafficking and the morphology, cell cycle and survival of PC12 cells
    Authors: Johansen, VBI;Hampson, E;Tsonou, E;Pantarelli, C;Chu, JY;Crossland, L;Okkenhaug, H;Massey, AJ;Hornigold, DC;Welch, HCE;Chetwynd, SA;
    Scientific reports
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. An S1P1 agonist, ASP4058, suppresses intracranial aneurysm through promoting endothelial integrity and blocking macrophage transmigration
    Authors: R Yamamoto, T Aoki, H Koseki, M Fukuda, J Hirose, K Tsuji, K Takizawa, S Nakamura, H Miyata, N Hamakawa, H Kasuya, K Nozaki, Y Hirayama, I Aramori, S Narumiya
    Br. J. Pharmacol., 2017-05-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Hypoxia augments outgrowth endothelial cell (OEC) sprouting and directed migration in response to sphingosine-1-phosphate (S1P).
    Authors: Williams P, Stilhano R, To V, Tran L, Wong K, Silva E
    PLoS ONE, 2015-04-15;10(4):e0123437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase delta but not SYK or BTK in chronic lymphocytic leukemia cells.
    Authors: Till K, Pettitt A, Slupsky J
    J Immunol, 2015-01-28;194(5):2439-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling.
    Authors: Gatfield J, Monnier L, Studer R, Bolli M, Steiner B, Nayler O
    Cell Signal, 2014-04-02;26(7):1576-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. New B-cell CD molecules.
    Authors: Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P
    Immunol. Lett., 2010-10-07;134(2):104-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010-05-19;24(10):3782-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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