Human S1P1/EDG-1 APC-conjugated Antibody

Catalog #: FAB2016A
1 Citation Datasheet / COA / SDS

Clone 218713 was used by HLDA to establish CD designation

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FAB2016A

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry.

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All S1P1/EDG-1 Antibodies

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Human S1P1/EDG-1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human S1P₁/EDG‑1 by flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 218713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human S1P₁/EDG‑1
Met1-Ser382
Accession # P21453

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of S1P<sub>1</sub>/EDG-1 antibody in CHO Chinese Hamster Cell Line Transfected with Human S<sub>1</sub>P1/EDG-1 and eGFP antibody by Flow Cytometry.

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Detection of S1P₁/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S₁P1/EDG-1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either (A) human S1P₁/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P₁/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB2016A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P₁, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P₁ signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name

Endothelial Differentiation Gene 1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Alternate Names

CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human S1P1/EDG-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1