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Human SALM2/LRFN1 Antibody

Catalog #: AF5669
1 Citation Datasheet / COA / SDS
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AF5669
AF5669-SP
Detection of Human SALM2 by Western Blot.
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Citations (1)
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Human SALM2/LRFN1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SALM2/LRFN1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SALM3 and rhSALM4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SALM2/LRFN1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SALM2 antibody by Western Blot. View Larger

Detection of Human SALM2 by Western Blot. Western blot shows lysates of human brain cortex and hipoocampus tissue and negative control lung and liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SALM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SALM2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM2/LRFN1

Synaptic adhesion-like molecule 2 (SALM2; also leucine-rich repeat and fibronectin type-III domain-containing protein 1 (Lrfn1) is a 105 kDa member of the Lrfn family of type I transmembrane glycoproteins (1‑2). Human SALM2 is synthesized as a 771 amino acid (aa) precursor that contains a 31 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 214 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 3). In addition, there are two potential sites for N-linked glycosylation. The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1‑3, but is absent in SALMs 4 and 5 (4). Mature human SALM2 shares 96% and 95% aa sequence identity with mature mouse and rat SALM2, respectively. In the developing mouse embryo, the temporal expression profile blot revealed a general increment of expression around E10.5 for SALM2. Northern blot analysis showed that in rats, SALM2 is strongly expressed in the adult brain and is also present in the adult testis (2). SALM2 localizes to both axons and dendrites (4). In addition, it co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons (2, 4). SALM2, like the other SALMs, promotes neurite outgrowth (4). In particular, SALM2 promotes an increase in the number of primary processes compared to controls and SALMs 1 and 5 (4). In addition, SALM2 is an important regulator of the differentiation of excitatory synapses (2). SALM2 may also be involved in synaptic maintenance and other cellular interactions (4).

References
  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Ko, J. et al. (2006) Neuron 50:233.
  3. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  4. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
Long Name
Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1
Entrez Gene IDs
57622 (Human); 365222 (Rat)
Alternate Names
KIAA1484; leucine rich repeat and fibronectin type III domain containing 1; leucine-rich repeat and fibronectin type III domain-containing protein 1; LRFN1; SALM2; synaptic adhesion-like molecule 2

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Citation for Human SALM2/LRFN1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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