Human SCF/c-kit Ligand Biotinylated Antibody

Catalog #: BAF255
2 Citations Datasheet / COA / SDS

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BAF255

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SCF/c-kit Ligand Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SCF/c-kit Ligand in ELISAs and Western blots. In sandwich immunoassays, approximately 55% cross-reactivity with recombinant feline SCF is observed, 20% cross-reactivity with recombinant canine SCF is observed, and less than 0.3% cross-reactivity with recombinant mouse SCF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SCF/c-kit Ligand (R&D Systems, Catalog # 255-SC)
Glu26-Ala189
Accession # P21583

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC)

Human SCF/c-kit Ligand Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human SCF/c‑kit Ligand Antibody (Catalog # MAB655)

Standard: Recombinant Human SCF Protein (Catalog # 255-SC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75%-83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

References

Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599.
Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
Kapur, R. et al. (2002) Blood 100:1287.
Wang, C-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
Martin, F.H. et al. (1990) Cell 63:203.
Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Long Name

Stem Cell Factor

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Alternate Names

c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human SCF/c-kit Ligand Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Growth hormone-induced stimulation of multilineage human hematopoiesis.
Authors: Hanley MB, Napolitano LA, McCune JM
Stem Cells, 2005-06-13;23(8):1170-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Inhibition by glucocorticoids of the interleukin-1beta-enhanced expression of the mast cell growth factor SCF.
Authors: Da Silva CA, 2019, Kassel O, Mathieu E, Massard G, Gasser B, Frossard N
2002-04-01;135(7):1634-40.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development