Human Semaphorin 7A Antibody

Catalog # Availability Size / Price Qty
MAB20681
MAB20681-SP
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Human Semaphorin 7A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Semaphorin 7A in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 310829
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human T cells treated with PHA
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 7A

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1‑4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1‑5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6‑10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9‑12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13). 

References
  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
  3. Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
  4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
  5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
  6. Suzuki, K. et al. (2007) Nature 446:680.
  7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Czopik, A.K. et al. (2006) Immunity 24:591.
  10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
  11. Mine, T. et al. (2000) Tissue Antigens 55:429.
  12. Kang, H.-R. et al. (2007) J. Exp. Med. 204:1083.
  13. Delorme, G. et al. (2005) Biol. Cell 97:589.
Entrez Gene IDs
8482 (Human); 20361 (Mouse)
Alternate Names
CD108 antigen; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH blood group antigen; JMH; John-Milton-Hargen human blood group Ag; MGC126692; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; Sema7A; SEMAK1; SEMAL; SEMALJohn Milton Hagen blood group H-Sema K1; Semaphorin 7A; semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; semaphorin-7A; semaphorin-K1; semaphorin-L

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