Human Serum Amyloid A1/A2 Antibody Summary
Arg19-Tyr122
Accession # P0DJI8
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30194).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Serum Amyloid A1 DuoSet ELISA Kit (Catalog # DY3019-05) for convenient development of a sandwich ELISA.Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Human Serum Amyloid A1/A2 ELISA Standard Curve. Recombinant human Serum Amyloid A1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30194) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB30196) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A1/A2
Human SAA1 is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), non‑glycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only seven amino acids. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA1 shares 72%, 82%, and 72% aa sequence identity with mature mouse, rabbit, and hamster SAA1, respectively.
Product Datasheets
Citation for Human Serum Amyloid A1/A2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity
Authors: U Smole, N Gour, J Phelan, G Hofer, C Köhler, B Kratzer, PA Tauber, X Xiao, N Yao, J Dvorak, L Caraballo, L Puerta, S Rosskopf, J Chakir, E Malle, AP Lane, WF Pickl, S Lajoie, M Wills-Karp
Nat. Immunol., 2020-06-22;21(7):756-765.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Serum Amyloid A1/A2 Antibody
There are currently no reviews for this product. Be the first to review Human Serum Amyloid A1/A2 Antibody and earn rewards!
Have you used Human Serum Amyloid A1/A2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
