Human sFRP-5 Antibody

Catalog #: MAB6266 Datasheet / COA / SDS

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MAB6266

Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody.

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Human sFRP-5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human sFRP-5 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 707128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human sFRP-5
Glu30-His317
Accession # Q5T4F7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize sFRP-5 inhibited Topflash reporter activity in the HEK293T human embryonic kidney cell line. The Neutralization Dose (ND₅₀) is typically 2-10 μg/mL in the presence of 5 μg/mL Recombinant Human sFRP-5 and 100 ng/mL Recombinant Mouse Wnt-3a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Topflash Reporter Activity Inhibited by sFRP‑5 and Neutralization by Human sFRP‑5 Antibody. Recombinant Human sFRP-5 (Catalog # 6266-SF) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity inhibited by Recombinant Human sFRP-5 (5 µg/mL) is neutralized (blue line) by increasing concentrations of Mouse Anti-Human sFRP-5 Monoclonal Antibody (Catalog # MAB6266) in the presence of 100 ng/mL Recombinant Mouse Wnt-3a. The ND₅₀ is typically 2-10 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: sFRP-5

Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP-2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down-regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial-mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down-regulated in mouse models of obesity and type 2 diabetes (10).

References

Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
van Amerongen, R. and R. Nusse (2009) Development 136:3205.
Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
Leaf, I. et al. (2006) Genesis 44:573.
Satoh, W. et al. (2008) Genesis 46:92.
Li, Y. et al. (2008) Genes Dev. 22:3050.
Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
Ouchi, N. et al. (2010) Science 329:454.
Veeck, J. et al. (2008) Carcinogenesis 29:991.
Zhao, C. et al. (2009) BMC Cancer 9:224.
Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

Long Name

Secreted Frizzled Related Protein 5

Entrez Gene IDs

6425 (Human); 54612 (Mouse)

Alternate Names

frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP5; sFRP-5