Human SGK1 Antibody Summary
Met1-Leu431
Accession # O00141
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SGK1 by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line non-transfected and transfected with human SGK1. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human SGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for SGK1 was detected at approximately 54 kDa (as indicated). For additional reference, recombinant human SGK1, SGK2, and SGK3 were included. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of SKG1 in Human Kidney. SKG1 was detected in paraffin-embedded sections of human kidney using Rabbit Anti-Human SGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) at 3 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (VC003). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SGK1
Serum- and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases. In addition to serum and glucocorticoids, insulin, IGF-I, osmotic shock, and mineralocorticoids have been demonstrated to activate SGK1. Expressed at low levels, SGK1 appears to function as a regulator of epithelial ion transport. Sustained high levels of SGK1 activation have been implicated in hypertension and diabetic nephropathy.
Product Datasheets
Citation for Human SGK1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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SGK1, a Serine/Threonine Kinase, Inhibits Prototype Foamy Virus Replication
Authors: Junshi Zhang, Chunhua Han, Zhenjie Xiong, Manman Qiu, Xiaopeng Tuo, Chenchen Wang et al.
Microbiology Spectrum
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