Human SHBG Antibody Summary
Leu30-His402
Accession # P04278
Applications
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB2656).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SHBG DuoSet ELISA Kit (Catalog # DY2656) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human SHBG ELISA Standard Curve. Recombinant Human SHBG protein was serially diluted 2-fold and captured by Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB2656) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB2656) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHBG
Human Sex Hormone Binding Globulin (SHBG), also known as ABP or androgen binding protein, is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 aa long laminin a chain G-like domains. Each SHBG monomer is a 47‑53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.
Product Datasheets
Citation for Human SHBG Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex
Authors: DL Lin, NA Cherepanov, L Bozzacco, MR MacDonald, R Gilmore, AW Tai
MBio, 2017-07-18;8(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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