Human SHBG Antibody Summary
Leu30-His402
Accession # P04278
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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SHBG in Human Liver. SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2656) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human SHBG by Western Blot. Western blot shows lysates of Human Liver. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SHBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2656) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SHBG at approximately ~37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHBG
Human SHBG (also known as ABP or androgen-binding protein) is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G-like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.
Product Datasheets
Citations for Human SHBG Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype
Authors: E Blommaert, R Péanne, NA Cherepanov, D Rymen, F Staels, J Jaeken, V Race, L Keldermans, E Souche, A Corveleyn, R Sparkes, K Bhattachar, C Devalck, R Schrijvers, F Foulquier, R Gilmore, G Matthijs
Proc. Natl. Acad. Sci. U.S.A., 2019-04-29;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Authors: Cherepanova N, Shrimal S, Gilmore R
J Cell Biol, 2014-08-18;206(4):525-39.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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