Human SHBG Biotinylated Antibody

Catalog #: BAF2656 Datasheet / COA / SDS

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BAF2656

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Human SHBG Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SHBG in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SHBG
Leu30-His402
Accession # P04278

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SHBG

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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SHBG in Human Liver. SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2656) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHBG

Human SHBG (also known as ABP or androgen-binding protein) is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G-like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Long Name

Sex Hormone-binding Globulin

Entrez Gene IDs

6462 (Human)

Alternate Names

ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin