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Human SOX9 Antibody

Catalog #: AF3075
78 Citations 4 Reviews Datasheet / COA / SDS
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AF3075
AF3075-SP
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Detection of Human SOX9 by Western Blot.
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Citations (78)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human SOX10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SOX9 antibody by Western Blot. View Larger

Detection of Human SOX9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SOX9 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry SOX9 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western Detection of Human SOX9 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human SOX9 by Simple WesternTM. Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name
Transcription Factor SOX9
Entrez Gene IDs
6662 (Human)
Alternate Names
campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9

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Citations for Human SOX9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

78 Citations: Showing 1 - 10
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  1. Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation
    Authors: J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas
    Cell Reports, 2023-01-05;42(1):111942.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice
    Authors: Y Wang, S Zhang, Z Lan, V Doan, B Kim, S Liu, M Zhu, VL Hull, S Rihani, CL Zhang, JA Gray, F Guo
    Cell Reports, 2022-12-20;41(12):111842.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. mTORC1 signaling facilitates differential stem cell differentiation to shape the developing murine lung and is associated with mitochondrial capacity
    Authors: K Zhang, E Yao, E Chuang, B Chen, EY Chuang, PT Chuang
    Nature Communications, 2022-11-25;13(1):7252.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Maturation and circuit integration of transplanted human cortical organoids
    Authors: O Revah, F Gore, KW Kelley, J Andersen, N Sakai, X Chen, MY Li, F Birey, X Yang, NL Saw, SW Baker, ND Amin, S Kulkarni, R Mudipalli, B Cui, S Nishino, GA Grant, JK Knowles, M Shamloo, JR Huguenard, K Deisseroth, SP Pa?ca
    Nature, 2022-10-12;610(7931):319-326.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: ICC
  5. WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome
    Authors: Y Tian, W Wang, S Lautrup, H Zhao, X Li, PWN Law, ND Dinh, EF Fang, HH Cheung, WY Chan
    Nature Communications, 2022-09-16;13(1):5456.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. SOX9 reprograms endothelial cells by altering the chromatin landscape
    Authors: BM Fuglerud, S Drissler, J Lotto, TL Stephan, A Thakur, R Cullum, PA Hoodless
    Nucleic Acids Research, 2022-08-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Wnt5a-Vangl1/2 signaling regulates the position and direction of lung branching through the cytoskeleton and focal adhesions
    Authors: K Zhang, E Yao, E Chuang, B Chen, EY Chuang, RF Volk, KL Hofmann, B Zaro, PT Chuang
    PloS Biology, 2022-08-26;20(8):e3001759.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. SHH and Notch regulate SOX9+ progenitors to govern arcuate POMC neurogenesis
    Authors: E Place, E Manning, DW Kim, A Kinjo, G Nakamura, K Ohyama
    Frontiers in Neuroscience, 2022-08-11;16(0):855288.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: ICC/IF
  9. Divergent transcriptional regulation of astrocyte reactivity across disorders
    Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
    Nature, 2022-05-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development
    Authors: T Gu, D Hao, J Woo, TW Huang, L Guo, X Lin, AG Guzman, A Tovy, C Rosas, M Jeong, Y Zhou, B Deneen, Y Huang, W Li, MA Goodell
    Nature Genetics, 2022-05-09;54(5):625-636.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Hedgehog Signaling Pathway Orchestrates Human Lung Branching Morphogenesis
    Authors: R Belgacemi, S Danopoulos, G Deutsch, I Glass, V Dormoy, S Bellusci, D Al Alam
    International Journal of Molecular Sciences, 2022-05-09;23(9):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Specification of CNS macrophage subsets occurs postnatally in defined niches
    Authors: T Masuda, L Amann, G Monaco, R Sankowski, O Staszewski, M Krueger, F Del Gaudio, L He, N Paterson, E Nent, F Fernández-, A Yamasaki, M Frosch, M Fliegauf, LFP Bosch, H Ulupinar, N Hagemeyer, D Schreiner, C Dorrier, M Tsuda, C Grothe, A Joutel, R Daneman, C Betsholtz, U Lendahl, KP Knobeloch, T Lämmermann, J Priller, K Kierdorf, M Prinz
    Nature, 2022-04-20;604(7907):740-748.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Age and Alzheimer's Disease-Related Oligodendrocyte Changes in Hippocampal Subregions
    Authors: L DeFlitch, E Gonzalez-F, I Crawley, SH Kang
    Frontiers in Cellular Neuroscience, 2022-04-07;16(0):847097.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Galectin-1 prevents pathological vascular remodeling in atherosclerosis and abdominal aortic aneurysm
    Authors: R Roldán-Mon, JM Pérez-Sáez, I Cerro-Pard, J Oller, D Martinez-L, E Nuñez, SM Maller, C Gutierrez-, N Mendez-Bar, JC Escola-Gil, JB Michel, M Mittelbrun, J Vázquez, LM Blanco-Col, GA Rabinovich, JL Martin-Ven
    Science Advances, 2022-03-16;8(11):eabm7322.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting
    Authors: AE Lewis, A Kuwahara, J Franzosi, JO Bush
    Cell Reports, 2022-03-15;38(11):110510.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  16. Localized astrogenesis regulates gyrification of the cerebral cortex
    Authors: Y Shinmyo, K Saito, T Hamabe-Hor, N Kameya, A Ando, K Kawasaki, TAD Duong, M Sakashita, J Roboon, T Hattori, T Kannon, K Hosomichi, M Slezak, MG Holt, A Tajima, O Hori, H Kawasaki
    Science Advances, 2022-03-11;8(10):eabi5209.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia
    Authors: T Xu, X Li, Y Guo, E Uhlin, L Holmberg, S Mitra, D Winn, A Falk, E Sundström
    EBioMedicine, 2022-02-16;77(0):103882.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Suspension culture promotes serosal mesothelial development in human intestinal organoids
    Authors: MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence
    Cell Reports, 2022-02-15;38(7):110379.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  19. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022-02-04;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. DM1 Transgenic Mice Exhibit Abnormal Neurotransmitter Homeostasis and Synaptic Plasticity in Association with RNA Foci and Mis-Splicing in the Hippocampus
    Authors: B Potier, L Lallemant, S Parrot, A Huguet-Lac, G Gourdon, P Dutar, M Gomes-Pere
    International Journal of Molecular Sciences, 2022-01-06;23(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Oligodendrocytes depend on MCL-1 to prevent spontaneous apoptosis and white matter degeneration
    Authors: AH Cleveland, A Romero-Mor, LA Azcona, M Herrero, VD Nikolova, S Moy, O Elroy-Stei, V Gama, TR Gershon
    Cell Death & Disease, 2021-12-06;12(12):1133.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. IL-33 induces orofacial neuropathic pain through Fyn-dependent phosphorylation of GluN2B in the trigeminal spinal nucleus caudalis
    Authors: Y Kimura, Y Hayashi, S Hitomi, D Ikutame, K Urata, I Shibuta, A Sakai, J Ni, K Iwata, M Tonogi, M Shinoda
    Brain, Behavior, and Immunity, 2021-10-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
    Authors: M Lattke, R Goldstone, JK Ellis, S Boeing, J Jurado-Arj, N Marichal, JI MacRae, B Berninger, F Guillemot
    Nature Communications, 2021-07-15;12(1):4335.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. GluA2 overexpression in oligodendrocyte progenitors promotes postinjury oligodendrocyte regeneration
    Authors: RR Khawaja, A Agarwal, M Fukaya, HK Jeong, S Gross, E Gonzalez-F, J Soboloff, DE Bergles, SH Kang
    Cell Reports, 2021-05-18;35(7):109147.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Orchestrated cellular, biochemical, and biomechanical optimizations endow platelet-rich plasma-based engineered cartilage with structural and biomechanical recovery
    Authors: K Wang, J Li, Y Wang, Y Wang, Y Qin, F Yang, M Zhang, H Zhu, Z Li
    Bioactive materials, 2021-04-10;6(11):3824-3838.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Human spinal GABA neurons alleviate spasticity and improve locomotion in rats with spinal cord injury
    Authors: C Gong, X Zheng, F Guo, Y Wang, S Zhang, J Chen, X Sun, SZA Shah, Y Zheng, X Li, Y Yin, Q Li, X Huang, T Guo, X Han, SC Zhang, W Wang, H Chen
    Cell Reports, 2021-03-23;34(12):108889.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  27. Long-term maturation of human cortical organoids matches key early postnatal transitions
    Authors: A Gordon, SJ Yoon, SS Tran, CD Makinson, JY Park, J Andersen, AM Valencia, S Horvath, X Xiao, JR Huguenard, SP Pa?ca, DH Geschwind
    Nature Neuroscience, 2021-02-22;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  28. ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response
    Authors: C Wang, M Zhang, G Garcia, E Tian, Q Cui, X Chen, G Sun, J Wang, V Arumugaswa, Y Shi
    Cell Stem Cell, 2021-01-04;28(2):331-342.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk
    Authors: SE Howden, SB Wilson, E Groenewege, L Starks, TA Forbes, KS Tan, JM Vanslambro, EM Holloway, YH Chen, S Jain, JR Spence, MH Little
    Cell Stem Cell, 2020-12-29;0(0):.
    Species: Human, Transgenic Human
    Sample Types: Organoid
    Applications: IHC
  30. ROBO2 signaling in lung development regulates SOX2/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia
    Authors: AN Gonçalves, J Correia-Pi, C Nogueira-S
    Respir Res, 2020-11-18;21(1):302.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Western Blot
  31. Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesis
    Authors: B Oulès, C Philippeos, J Segal, M Tihy, M Vietri Rud, AM Cujba, PA Grange, S Quist, K Natsuga, L Deschamps, N Dupin, G Donati, FM Watt
    Nat Commun, 2020-10-20;11(1):5067.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  32. DSCAM regulates delamination of neurons in the developing midbrain
    Authors: N Arimura, M Okada, S Taya, KI Dewa, A Tsuzuki, H Uetake, S Miyashita, K Hashizume, K Shimaoka, S Egusa, T Nishioka, Y Yanagawa, K Yamakawa, YU Inoue, T Inoue, K Kaibuchi, M Hoshino
    Science Advances, 2020-09-02;6(36):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Regional Variation in Epidermal Susceptibility to UV-Induced Carcinogenesis Reflects Proliferative Activity of Epidermal Progenitors
    Authors: E Roy, HY Wong, R Villani, T Rouille, B Salik, SL Sim, V Murigneux, MS Stark, JL Fink, HP Soyer, G Walker, JG Lyons, N Saunders, K Khosrotehr
    Cell Rep, 2020-06-02;31(9):107702.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. Overexpression of MIG-6 in the cartilage induces an osteoarthritis-like phenotype in mice
    Authors: M Bellini, MA Pest, M Miranda-Ro, L Qin, JW Jeong, F Beier
    Arthritis Res. Ther., 2020-05-19;22(1):119.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Redundant and additive functions of the four Lef/Tcf transcription factors in lung epithelial progenitors
    Authors: KN Gerner-Mau, H Akiyama, J Chen
    Proc. Natl. Acad. Sci. U.S.A., 2020-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development
    Authors: T Shoneye, AT Orrego, R Jarvis, Y Men, MSR Chiang, Y Yang
    Front Neurosci, 2020-05-08;14(0):435.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Embryonic microglia influence developing hypothalamic glial populations
    Authors: CM Marsters, D Nesan, R Far, N Klenin, QJ Pittman, DM Kurrasch
    J Neuroinflammation, 2020-05-06;17(1):146.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  38. Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone
    Authors: KL Adams, G Riparini, P Banerjee, M Breur, M Bugiani, V Gallo
    Nat Commun, 2020-05-01;11(1):2138.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation
    Authors: A Fleischer, S Vallejo-Dí, JM Martín-Fer, A Sánchez-Gi, M Castresana, A Del Pozo, A Esquisabel, S Ávila, JL Castrillo, E Gaínza, JL Pedraz, M Viñas, D Bachiller
    Mol Ther Methods Clin Dev, 2020-04-18;17(0):858-870.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Injured adult neurons regress to an embryonic transcriptional growth state
    Authors: GHD Poplawski, R Kawaguchi, E Van Nieker, P Lu, N Mehta, P Canete, R Lie, I Dragatsis, JM Meves, B Zheng, G Coppola, MH Tuszynski
    Nature, 2020-04-15;581(7806):77-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
    Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
    Cell Rep, 2020-04-07;31(1):107471.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Individual Limb Muscle Bundles Are Formed through Progressive Steps Orchestrated by Adjacent Connective Tissue Cells during Primary Myogenesis
    Authors: L Besse, CJ Sheeba, M Holt, M Labuhn, S Wilde, E Feneck, D Bell, A Kucharska, MPO Logan
    Cell Rep, 2020-03-10;30(10):3552-3565.e6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  43. Temporospatial Expression of Fgfr1 and 2 During Lung Development, Homeostasis, and Regeneration
    Authors: T Yuan, K Klinkhamme, H Lyu, S Gao, J Yuan, S Hopkins, JS Zhang, SP De Langhe
    Front Pharmacol, 2020-03-02;11(0):120.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  44. Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice
    Authors: K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo
    Dis Model Mech, 2019-11-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  45. Development of glial restricted human neural stem cells for oligodendrocyte differentiation in vitro and in vivo
    Authors: S Biswas, SH Chung, P Jiang, S Dehghan, W Deng
    Sci Rep, 2019-06-21;9(1):9013.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  46. Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
    Authors: OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti
    Nat Protoc, 2019-05-20;14(6):1884-1925.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
    Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
    Nat. Cell Biol., 2019-04-01;21(4):430-441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  48. Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion
    Authors: MJ Castle, Y Cheng, A Asokan, MH Tuszynski
    Sci Adv, 2018-11-14;4(11):eaau9859.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  49. Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes
    Authors: JR Jones, L Kong, MG Hanna, B Hoffman, R Krencik, R Bradley, T Hagemann, J Choi, M Doers, M Dubovis, MA Sherafat, A Bhattachar, C Kendziorsk, A Audhya, A Messing, SC Zhang
    Cell Rep, 2018-10-23;25(4):947-958.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  50. 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling
    Authors: Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que
    Cell Stem Cell, 2018-09-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  51. Complex bile duct network formation within liver decellularized extracellular matrix hydrogels
    Authors: PL Lewis, J Su, M Yan, F Meng, SS Glaser, GD Alpini, RM Green, B Sosa-Pined, RN Shah
    Sci Rep, 2018-08-15;8(1):12220.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  52. In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus
    Authors: JM Rosin, DM Kurrasch
    J Neuroinflammation, 2018-06-12;15(1):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  53. Does human endometrial LGR5 gene expression suggest the existence of another hormonally regulated epithelial stem cell niche?
    Authors: N Tempest, AM Baker, NA Wright, DK Hapangama
    Hum. Reprod., 2018-06-01;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  54. Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors
    Authors: II Aigha, B Memon, AK Elsayed, EM Abdelalim
    Stem Cell Res Ther, 2018-04-03;9(1):83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  55. Glutamate transporter Slc1a3 mediates inter-niche stem cell activation during skin growth
    Authors: B Reichenbac, J Classon, T Aida, K Tanaka, M Genander, C Göritz
    EMBO J., 2018-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  56. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
    Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
    Dev. Cell, 2018-03-26;44(6):679-693.e5.
    Species: Human, Mouse
    Sample Types: Organoid, Whole Tissue
    Applications: IHC-Fr
  57. Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells
    Authors: M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo
    Stem Cell Res Ther, 2018-03-15;9(1):67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Restorative effects of human neural stem cell grafts on the primate spinal cord
    Authors: ES Rosenzweig, JH Brock, P Lu, H Kumamaru, EA Salegio, K Kadoya, JL Weber, JJ Liang, R Moseanko, S Hawbecker, JR Huie, LA Havton, YS Nout-Lomas, AR Ferguson, MS Beattie, JC Bresnahan, MH Tuszynski
    Nat. Med., 2018-02-26;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  59. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
    Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
    Stem Cell Res Ther, 2018-01-23;9(1):15.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  60. Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation
    Authors: T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino
    J. Neurosci., 2018-01-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  61. Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preB�tzinger complex
    Authors: A Kottick, CA Martin, CA Del Negro
    Physiol Rep, 2017-06-01;5(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos
    Authors: WJ Lee, S Chatterjee, SP Yap, SL Lim, X Xing, P Kraus, W Sun, X Hu, V Sivakamasu, HY Chan, PR Kolatkar, S Prabhakar, T Lufkin
    Biomed Res Int, 2017-05-28;2017(0):8932583.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  63. SOX9 is an astrocyte-specific nuclear marker in the adult brain outside the neurogenic regions
    Authors: W Sun, A Cornwell, J Li, S Peng, MJ Osorio, NA Su Wanga, A Benraiss, N Lou, SA Goldman, M Nedergaard
    J. Neurosci, 2017-03-23;0(0):.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC
  64. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis
    Authors: C Lin, E Yao, K Zhang, X Jiang, S Croll, K Thompson-P, PT Chuang
    Elife, 2017-03-21;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  65. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate
    Authors: Emma L Rawlins
    Development, 2016-08-30;143(20):3686-3699.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  66. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2015-11-19;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  67. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
    Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
    Stem Cell Reports, 2015-02-05;4(3):419-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  68. In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
    Authors: Chatterjee S, Sivakamasundari V, Yap S, Kraus P, Kumar V, Xing X, Lim S, Sng J, Prabhakar S, Lufkin T
    BMC Genomics, 2014-12-05;15(0):1072.
    Species: Mouse
    Sample Types: Chromatin
    Applications: ChIP
  69. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
    Authors: Zhang X, Westerhoff M, Hart J
    Mod Pathol, 2014-11-21;28(5):654-61.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  70. The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
    Authors: Mahoney J, Mori M, Szymaniak A, Varelas X, Cardoso W
    Dev Cell, 2014-07-17;30(2):137-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  71. Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
    Nat Commun, 2014-05-29;5(0):3923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  72. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  73. The vanishing testis: a histomorphologic and clinical assessment.
    Authors: Antic T, Hyjek EM, Taxy JB
    Am. J. Clin. Pathol., 2011-12-01;136(6):872-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  74. Identification of SOX3 as an XX male sex reversal gene in mice and humans.
    Authors: Sutton E, Hughes J, White S
    J. Clin. Invest., 2010-12-22;121(1):328-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  75. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010-04-01;93(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  76. Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.
    Authors: Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B
    Oncogene, 2009-06-22;28(35):3121-31.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  77. A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
    Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
    Stem Cells, 2009-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).
    Authors: Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH
    J. Pathol., 2008-05-01;215(1):31-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human SOX9 Antibody
By Anonymous on 07/14/2020
Application: ICC/IF Sample Tested: Embryo tissue Species: Mouse
Immunocytochemistry/Immunofluorescence Human SOX9 Antibody AF3075
Human SOX9 Antibody
By Chang Xie on 03/20/2019
Sample Tested: Embryonic lung Species: Mouse

PFA fixed, sectioned, PBS washed, treated for 5 min in cold Methanol. Worked great!

Human SOX9 Antibody
By Preethi Vijayaraj on 09/27/2017
Application: ICC/IF Sample Tested: iPSC differentiated epithelial cells Species: Human
Immunocytochemistry/Immunofluorescence Human SOX9 Antibody AF3075
Human SOX9 Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: stem cells Species: Human

For immunohistochemistry & immunocytochemistry application.