Human Spi-B Antibody

Catalog #: MAB7576 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7576
MAB7576-SP

Detection of Human Spi‑B by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Spi-B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Spi-B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spi-B is observed.

Source

Monoclonal Mouse IgG_2B Clone # 769653

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Spi-B
Ala7-Leu115
Accession # Q01892

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Spi‑B by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Spi-B Monoclonal Antibody (Catalog # MAB7576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Spi-B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spi-B

Spi-B is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. The 262 amino acid (aa) human Spi-B contains a dual transactivation region (aa 1-62) and an ets DNA-binding domain (aa 169-252). One 177 aa variant diverges after aa 162. Over aa 7-115, human Spi-B shares 72% and 70% aa identity with mouse and rat Spi-B, respectively.

Long Name

Transcription Factor Spi [Spleen FFV Proviral Integration Oncogene] B

Entrez Gene IDs

6689 (Human); 272382 (Mouse); 499146 (Rat)

Alternate Names

Spi-B transcription factor (Spi-1/PU.1 related); SpiB; Spi-B; transcription factor Spi-B