Human SR-AI/MSR PE-conjugated Antibody Summary
Lys77-Leu451
Accession # P21757
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of SR-AI/MSR antibody in THP-1 Human Cell Line antibody by Flow Cytometry. Detection of SR-AI/MSR antibody in THP-1 Human Cell Line antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/SRAI_FAB2708P_Flow_Cytometry_13217.jpg)
Detection of SR‑AI/MSR in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line activated with PMA and Ca2+ ionomycin was stained with Mouse Anti-Human SR-AI/MSR PE-conjugated Monoclonal Antibody (Catalog # FAB2708P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
![](https://resources.rndsystems.com/images/datasheets/fab2708p_human-sr-ai-msr1-phycoerythrin-mab-clone-351615-flow-cytometry-1112023102610.jpg)
Detection of SR‑AI/MSR in M2 macrophages cells by Flow Cytometry. M2 macrophages cells treated with 50 ng/mL Recombinant Human M-CSF Protein (216-MC) for 6 days followed by an overnight polarization with 20 ng/mL Recombinant Human IL-4 Protein (204-IL) and 20 ng/mL Recombinant Human IL-13 Protein (213-ILB) were stained with Mouse Anti-Human SR‑AI/MSR PE‑conjugated Monoclonal Antibody (Catalog # FAB2708P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SR-AI/MSR
The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.
- Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
- Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
- Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
- Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
- Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
- Naito, M. et al. (1992) Am. J. Pathol. 141:591.
- Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
- Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
- Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
- Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
- Peiser, L. et al. (2000) Inf. Immun. 68:1953.
Product Datasheets
Citations for Human SR-AI/MSR PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Gastric cancer-derived mesenchymal stromal cells trigger M2 macrophage polarization that promotes metastasis and EMT in gastric cancer
Authors: W Li, X Zhang, F Wu, Y Zhou, Z Bao, H Li, P Zheng, S Zhao
Cell Death Dis, 2019-12-04;10(12):918.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages
Authors: OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura
Redox Biol, 2017-11-22;15(0):1-11.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer
Authors: B Zhang, M Cao, Y He, Y Liu, G Zhang, C Yang, Y Du, J Xu, J Hu, F Gao
J Cancer, 2017-09-29;8(17):3522-3530.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
J. Immunol., 2017-07-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased circulating M2-like monocytes in patients with breast cancer
Authors: B Zhang, M Cao, Y He, Y Liu, G Zhang, C Yang, Y Du, J Xu, J Hu, F Gao
Tumour Biol., 2017-06-01;39(6):1010428317711.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids
Authors: AB Chinen, CM Guan, CH Ko, CA Mirkin
Small, 2017-02-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effect of apoptotic cell recognition on macrophage polarization and mycobacterial persistence.
Authors: de Oliveira Fulco T, Andrade P, de Mattos Barbosa M, Pinto T, Ferreira P, Ferreira H, da Costa Nery J, Real S, Borges V, Moraes M, Sarno E, Sampaio E, Pinheiro R
Infect Immun, 2014-07-14;82(9):3968-78.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Oxidative stress decreases functional airway mannose binding lectin in COPD.
Authors: Tran, Hai B, Ahern, Jessica, Hodge, Greg, Holt, Phillip, Dean, Melinda, Reynolds, Paul N, Hodge, Sandra
PLoS ONE, 2014-06-05;9(6):e98571.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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