Human SREC-II/SCARF2 Antibody

Catalog #: AF2527 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2527
AF2527-SP

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SREC-II/SCARF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects SREC‑II/SCARF2 in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SREC-I is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SREC‑II/SCARF2
Ala42-Gly442
Accession # Q96GP6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human SREC-II Fc Chimera (Catalog # 2527-SR)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 2 μg/mL of Recombinant Human SREC-II Fc Chimera (Catalog # 2527-SR) to immobilized human acetylated low-density lipoproteins (AcLDL) coated at 2 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SREC-II/SCARF2

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL(OxLDL) (1‑3). Family members are classified alphabetically. In general, they play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). Unlike SREC-I, SREC-II is somewhat unique in that it doesn’t show expected binding to modified LDL (5). The full length cDNA for human SREC-II encodes an 870 amino acid (aa) type I transmembrane protein which contains a 43 aa signal peptide, a 398 aa extracellular region, a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain (5, 6). Depending upon the reference, the extracellular region contains 7‑10 EGF-like repeats, with a cytoplasmic domain that is rich in serine and proline in its N-terminal half and glycine in its C-terminal segment (5, 6). There is at least one alternate splice variant that shows a 5 aa deletion in the juxtamembrane region of the cytoplasmic domain (6, 7). The deletion does not change the reading frame as the distal 390 amino acids are identical in the two isoforms. The Genbank suggests another variant is possible involving amino acids 750‑768 of the precursor. Again, this doesn’t seem to change the reading frame as amino acids downstream of this are unchanged (6, 8). SREC-II is expressed by endothelial cells and vascular smooth muscle cells (5, 9). In the extracellular region human SREC-II is 95% aa identical to mouse SREC-II. The extracellular regions of human SREC-II and SREC-I are 53% aa identical. Notably, more than serving as a scavenger receptor, SREC-II would seem to form heterophilic interactions with SREC-I during cell-to-cell aggregation (5).

References

Horiuchi, S. et al. (2003) Amino Acids 25:283.
Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
Ishii, J. et al. (2002) J. Biol. Chem. 277:39696.
GenBank Accession # Q96GP6.
GenBank Accession # NP_699165.
GenBank Accession # NP_878315.
Sukhanov, S. et al. (2003) Biochem. Biophys. Res. Commun. 306:443.

Long Name

Scavenger Receptor Expressed by Endothelial Cells 2

Entrez Gene IDs

91179 (Human)

Alternate Names

HUMZD58C02; SCARF2; scavenger receptor class F, member 2; Scavenger receptor expressed by endothelial cells 2 protein; SREC2NSR1; SRECII; SREC-II; SREC-IIscavenger receptor class F member 2; SRECRP-1; SREPCR