Human ST2/IL-33R APC-conjugated Antibody Summary
Lys19-Phe328
Accession # BAA02233
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ST2/IL-33 R in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R APC-conjugated Polyclonal Antibody (Catalog # FAB5231A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ST2/IL-33R
ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33's ability to signal through transmembrane ST2 (10, 13‑15).
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Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
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Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
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Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
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Li, H. et al. (2000) Genomics 67:284.
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Schmitz, J. et al. (2005) Immunity 23:479.
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Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
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Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
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Weinberg, E.O. et al. (2002) Circulation 106:2961.
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Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
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Palmer, G. et al. (2008) Cytokine 42:358.
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Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
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Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
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Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
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Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
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Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Product Datasheets
Citations for Human ST2/IL-33R APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Organoids capture tissue-specific innate lymphoid cell development in mice and humans
Authors: GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves
Cell Reports, 2022-08-30;40(9):111281.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-33 receptor expression on myeloid and plasmacytoid dendritic cells after allergen challenge in patients with allergic rhinitis
Authors: YQ Peng, DH Chen, ZB Xu, SB Fang, BX He, XQ Liu, CA Akdis, QL Fu
International immunopharmacology, 2021-10-12;101(0):108233.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytomegalovirus Infection Impairs the Mobilization of Tissue-Resident Innate Lymphoid Cells into the Peripheral Blood Compartment in Response to Acute Exercise
Authors: E Cho, B Theall, J Stampley, J Granger, NM Johannsen, BA Irving, G Spielmann
Viruses, 2021-08-03;13(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Expressions of TSLP, IL-33, and IL-17A in Monocyte Derived Dendritic Cells from Asthma and COPD Patients are Related to Epithelial-Macrophage Interactions
Authors: M Paplinska-, P Misiukiewi, M Proboszcz, P Nejman-Gry, K Gorska, R Krenke
Cells, 2020-08-22;9(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased KLRG1 and PD-1 expression on CD8 T lymphocytes in TB-IRIS
Authors: O Goovaerts, M Massinga-L, P Ondoa, A Ceulemans, W Worodria, H Mayanja-Ki, R Colebunder, L Kestens
PLoS ONE, 2019-04-25;14(4):e0215991.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL?33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin ?4?1 and CD62L
Authors: XH Wang, W Liu, DX Fan, WT Hu, MQ Li, XY Zhu, LP Jin
Mol Med Rep, 2017-07-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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