Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody

Catalog #: MAB67151 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB67151
MAB67151-SP

GalNAc alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 in MDA‑MB‑231 Human Cell Line.

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Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 719508

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SGalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5
Gly30-Phe336
Accession # Q9BVH7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GalNAc alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 in MDA‑MB‑231 Human Cell Line. GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB67151) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4(NeuAc alpha 2,3)Gal beta 1,4Glc beta 1-Cer) from GM1b (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4Gal beta 1,4Glc beta 1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.

References

Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
Bos, P.D. et al. (2009) Nature 459:1005.
Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.

Long Name

ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 5

Entrez Gene IDs

81849 (Human); 26938 (Mouse)

Alternate Names

alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase E; EC 2.4.99; EC 2.4.99.-; EC 2.4.99.7; GalNAc alpha-2,6-sialyltransferase V; GD1 alpha synthase; MGC3184; sialyltransferase 7 (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase) E; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) E; Sialyltransferase 7E; SIAT7E; SIAT7-E; SIAT7Ealpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase V; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5; ST6 neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5; ST6GalNAc V; ST6GALNAC5; ST6GalNAcV; ST6GalNAcValpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5