Human Stabilin-2 Antibody

Catalog #: AF3645
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3645
AF3645-SP

Detection of Stabilin‑2 in K562 Human Cell Line by Flow Cytometry.

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All Stabilin-2 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Stabilin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Stabilin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Stabilin-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Stabilin-2
Gly2198-Val2295
Accession # Q8WWQ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Stabilin‑2 (Catalog # 3645-S2)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Stabilin-2 antibody in K562 Human Cell Line antibody by Flow Cytometry.

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Detection of Stabilin‑2 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Sheep Anti-Human Stabilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3645, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stabilin-2

Stabilin-2, also known as HARE (hyaluronan receptor for endocytosis) and FEEL-2 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 2), is a type I transmembrane multi-domain protein that is the closest homolog of stabilin-1 (1, 2). It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG-6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin-2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrin-coated pits (4). As a recycling receptor, Stabilin-2 cycles from the membrane to clathrin-coated pits each 10‑15 minutes, with about two thirds of the protein found within the endocytic system at any one time (4). The link domain binds and mediates the systemic clearance of hyaluronan (HA) (2‑6). Within the link domain (aa 2198‑2295), human Stabilin-2 shares 86% and 89% aa identity with mouse and rat Stabilin-2, respectively. Total human HA is about 15 grams, of which about 5 grams are cleared each day (5). Stabilin-2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation end-product (AGE), collagen N-terminal propeptides and acetylated LDL (4‑7). Human Stabilin-2 mRNA encodes a 2251 aa, 315 kDa protein that produces an isoform of 190 kDa through proteolytic cleavage (2, 6). The 190 kDa form lacks some N-terminal fasciclin and EGF-like domains, but shows similar activities when compared with the 315 kDa form (2, 6).

References

Politz, O. et al. (2002) Biochem. J. 362:155.
Zhou, B. et al. (2003) Glycobiology 13:339.
Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.

Entrez Gene IDs

55576 (Human); 192188 (Mouse); 282580 (Rat)

Alternate Names

CD44-like precursor FELL; DKFZP434E0321; FAS1 EGF-like and X-link domain containing adhesion molecule-2; FAS1 EGF-like and X-link domain-containing adhesion molecule 2; fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-2; Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 2; FEEL2; FEEL-2; FELE-2; FELL; FELL-2; FEX2; HARE; HAREFELL2; hepatic hyaluronan clearance receptor; Hyaluronan receptor for endocytosis; hyaluronic acid receptor for endocytosis; STAB2; STAB-2; stabilin 2; Stabilin2; Stabilin-2

Product Datasheets

Product Datasheet

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Citations for Human Stabilin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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