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Human Stanniocalcin 1/STC-1 Antibody

Catalog #: AF2958
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2958
AF2958-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Stanniocalcin 1/STC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Stanniocalcin 1/STC-1 in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant human STC-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Stanniocalcin 1
Thr18-Ala247
Accession # P52823
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Stanniocalcin 1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stanniocalcin 1/STC-1

Stanniocalcin-1 (STC-1) is a secreted 35 kDa disulfide-linked homodimer that is related to a calcium-regulating hormone in fish. Mature human Stanniocalcin-1 is 230 aa in length, and contains five intrachain disulfide bonds plus one interchain disulfide bond. Circulating Stanniocalcin-1 is both glycosylated and phosphorylated. Mature human Stanniocalcin-1 is 98%, 97%, 95% and 97% aa identical to mature canine, mouse, bovine and rat Stanniocalcin-1, respectively. Stanniocalcin-1 promotes phosphorus absorption and blocks chemokine-induced chemotaxis.

Entrez Gene IDs
6781 (Human)
Alternate Names
Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1

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Citations for Human Stanniocalcin 1/STC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Stanniocalcin-1 Potently Inhibits the Proteolytic Activity of the Metalloproteinase Pregnancy-associated Plasma Protein-A.
    Authors: Kloverpris S, Mikkelsen J, Pedersen J, Jepsen M, Laursen L, Petersen S, Oxvig C
    J Biol Chem, 2015-07-20;290(36):21915-24.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Mesenchymal stromal cells protect cancer cells from ROS-induced apoptosis and enhance the Warburg effect by secreting STC1.
    Authors: Ohkouchi S, Block GJ, Katsha AM
    Mol. Ther., 2011-12-06;20(2):417-23.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  3. Production of calcium maintenance factor Stanniocalcin-1 (STC1) by the equine endometrium during the early pregnant period.
    Authors: Kikuchi M, Nakano Y, Nambo Y, Haneda S, Matsui M, Miyake Y, Macleod JN, Nagaoka K, Imakawa K
    J. Reprod. Dev., 2010-12-03;57(2):203-11.
    Species: Equine
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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