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Human STIM1 Antibody

Catalog #: MAB69761 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB69761
MAB69761-SP
STIM1 in Human Brain.
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Human STIM1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human STIM1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human STIM2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 705129
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human STIM1
Leu23-Thr182
Accession # Q13586
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry STIM1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

STIM1 in Human Brain. STIM1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human STIM1 Monoclonal Antibody (Catalog # MAB69761) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STIM1

STIM-1 (stromal interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the plasma membrane Ca2+ release-activated Ca2+ (CRAC) channel Orai1, increasing Ca2+ influx. The human STIM-1 extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2+-binding motif and a SAM (sterile a-motif) multimerization domain. A potential isoform is truncated at aa 491. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen, human STIM1 shares 95% amino acid identity with mouse and rat STIM1.

Long Name
Stromal Interaction Molecule 1
Entrez Gene IDs
6786 (Human); 20866 (Mouse); 361618 (Rat)
Alternate Names
GOK; GOKD11S4896E; STIM1; stromal interaction molecule 1

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Human STIM1 Antibody
By Anonymous on 03/03/2022
Application: IHC Sample Tested: Lung cancer tissue Species: Human
Immunohistochemistry Human STIM1 Antibody MAB69761