Human Syndecan-1/CD138 APC-conjugated Antibody Summary
Gln18-Glu251
Accession # NP_002988
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Syndecan‑1/CD138 in RPMI 8226 Human Cell Line by Flow Cytometry. RPMI 8226 human multiple myeloma cell line was stained with Rat Anti-Human Syndecan-1/CD138 APC-conjugated Monoclonal Antibody (Catalog # FAB2780A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Syndecan-1/CD138
Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of Heparan Sulfate (HS) and chondroitin sulfate Glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120-200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65-71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4-8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10-12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4).
- Tkachenko, E. et al. (2005) Circ. Res. 96:488.
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- Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
- Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
- Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
- Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
- Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
- Vanhoutte, D. et al. (2007) Circulation 115:475.
- Li, Q. et al. (2002) Cell 111:635.
- Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
- Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
- Derksen, P.W.B. et al. (2002) Blood 99:1405.
- Su, G. et al. (2007) J. Biol. Chem. 282:14906.
Product Datasheets
Citations for Human Syndecan-1/CD138 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Contribution of Syndecans to the Cellular Entry of SARS-CoV-2
Authors: A Hudák, A Letoha, L Szilák, T Letoha
International Journal of Molecular Sciences, 2021-05-19;22(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Contribution of syndecans to cellular internalization and fibrillation of amyloid-?(1-42)
Authors: T Letoha, A Hudák, E Kusz, A Pettkó-Sza, I Domonkos, K Jósvay, M Hofmann-Ap, L Szilák
Sci Rep, 2019-02-04;9(1):1393.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus
Authors: U Ellinghaus, A Cortini, CL Pinder, GL Friec, C Kemper, TJ Vyse
Eur. J. Immunol., 2017-05-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.
Authors: Hulse K, Norton J, Suh L, Zhong Q, Mahdavinia M, Simon P, Kern R, Conley D, Chandra R, Tan B, Peters A, Grammer L, Harris K, Carter R, Kato A, Schleimer R
J Allergy Clin Immunol, 2013-03-06;131(4):1075-83, 1083.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Canonical and noncanonical Hedgehog pathway in the pathogenesis of multiple myeloma.
Authors: Blotta, Simona, Jakubikova, Jana, Calimeri, Teresa, Roccaro, Aldo M, Amodio, Nicola, Azab, Abdel Ka, Foresta, Umberto, Mitsiades, Constant, Rossi, Marco, Todoerti, Katia, Molica, Stefano, Morabito, Fortunat, Neri, Antonino, Tagliaferri, Piersand, Tassone, Pierfran, Anderson, Kenneth, Munshi, Nikhil C
Blood, 2012-07-20;120(25):5002-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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